Universität zu Köln

Gehlert, Sebastian ORCID: 0000-0001-6940-3084, Klinz, Franz Josef, Willkomm, Lena, Schiffer, Thorsten, Suhr, Frank and Bloch, Wilhelm (2016). Intense Resistance Exercise Promotes the Acute and Transient Nuclear Translocation of Small Ubiquitin-Related Modifier (SUMO)-1 in Human Myofibres. Int. J. Mol. Sci., 17 (5). BASEL: MDPI. ISSN 1422-0067

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Abstract

Protein sumoylation is a posttranslational modification triggered by cellular stress. Because general information concerning the role of small ubiquitin-related modifier (SUMO) proteins in adult skeletal muscle is sparse, we investigated whether SUMO-1 proteins will be subjected to time-dependent changes in their subcellular localization in sarcoplasmic and nuclear compartments of human type I and II skeletal muscle fibers in response to acute stimulation by resistance exercise (RE). Skeletal muscle biopsies were taken at baseline (PRE), 15, 30, 60, 240 min and 24 h post RE from 6 male subjects subjected to a single bout of one-legged knee extensions. SUMO-1 localization was determined via immunohistochemistry and confocal laser microscopy. At baseline SUMO-1 was localized in perinuclear regions of myonuclei. Within 15 and up to 60 min post exercise, nuclear SUMO-1 localization was significantly increased (p < 0.01), declining towards baseline levels within 240 min post exercise. Sarcoplasmic SUMO-1 localization was increased at 15 min post exercise in type I and up to 30 min post RE in type II myofibres. The changing localization of SUMO-1 proteins acutely after intense muscle contractions points to a role for SUMO proteins in the acute regulation of the skeletal muscle proteome after exercise.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Gehlert, Sebastian UNSPECIFIED orcid.org/0000-0001-6940-3084 UNSPECIFIED Klinz, Franz Josef UNSPECIFIED UNSPECIFIED UNSPECIFIED Willkomm, Lena UNSPECIFIED UNSPECIFIED UNSPECIFIED Schiffer, Thorsten UNSPECIFIED UNSPECIFIED UNSPECIFIED Suhr, Frank UNSPECIFIED UNSPECIFIED UNSPECIFIED Bloch, Wilhelm UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-276315
DOI:	10.3390/ijms17050646
Journal or Publication Title:	Int. J. Mol. Sci.
Volume:	17
Number:	5
Date:	2016
Publisher:	MDPI
Place of Publication:	BASEL
ISSN:	1422-0067
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language HUMAN SKELETAL-MUSCLE; GTPASE-ACTIVATING PROTEIN; MITOCHONDRIAL BIOGENESIS; SIGNALING RESPONSES; SUMO-1 MODIFICATION; TIME-COURSE; C-JUN; ADAPTATION; MECHANISMS; INCREASES Multiple languages Biochemistry & Molecular Biology; Chemistry, Multidisciplinary Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/27631