Universität zu Köln

Aldeyarbi, Hebatalla M. and Karanis, Panagiotis (2016). The fine structure of sexual stage development and sporogony of Cryptosporidium parvum in cell-free culture. Parasitology, 143 (6). S. 749 - 762. NEW YORK: CAMBRIDGE UNIV PRESS. ISSN 1469-8161

Full text not available from this repository.

Abstract

The sexual stages and new oocysts development of Cryptosporidium parvum were investigated in a cell-free culture system using transmission electron microscopy (TEM). Sexual development was extremely rapid after inoculation of oocysts into the medium. The process began within 1/2-12 h and was completed with new oocyst formation 120 h post-inoculation. The macrogamonts were bounded by two membranes and had amylopectin granules and two distinct types of wall-forming bodies. The microgamonts had a large nucleus showing lobe projections and condensation of chromatin, giving rise to peripherally budding microgametes. The microgametes contained a large area of granular substance containing groups of microtubules surrounding the electron-dense nucleus. In some instances, the dividing microgamy was observed in cell-free cultures with no preceding merogonic process. Fertilization was observed with the bullet-shaped microgamete penetrating an immature macrogamont at 24 and 216 h. The new thin-and thick-walled oocysts had a large residuum with polysaccharide granules and sporogony noted inside these oocysts. Novel immature four-layer walled thick oocysts with irregular knob-like protrusions on the outer layer resembling the immature Eimeria oocysts were also observed. The present study confirms the gametogony and sporogony of C. parvum in cell-free culture and describes their ultra-structure for the first time.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Aldeyarbi, Hebatalla M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Karanis, Panagiotis UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-276513
DOI:	10.1017/S0031182016000275
Journal or Publication Title:	Parasitology
Volume:	143
Number:	6
Page Range:	S. 749 - 762
Date:	2016
Publisher:	CAMBRIDGE UNIV PRESS
Place of Publication:	NEW YORK
ISSN:	1469-8161
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language TOXOPLASMA-GONDII; HOST-CELL; ULTRASTRUCTURAL-CHANGES; EXCYSTING SPOROZOITES; ENDOGENOUS STAGES; SMALL-INTESTINE; SARCOCYSTIS SP; OOCYST WALLS; LIFE-CYCLE; ELECTRON Multiple languages Parasitology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/27651