Universität zu Köln

Scholz, Paul, Kalbe, Benjamin, Jansen, Fabian, Altmueller, Janine, Becker, Christian, Mohrhardt, Julia, Schreiner, Benjamin, Gisselmann, Guenter, Hatt, Hanns and Osterloh, Sabrina (2016). Transcriptome Analysis of Murine Olfactory Sensory Neurons during Development Using Single Cell RNA-Seq. Chem. Senses, 41 (4). S. 313 - 324. OXFORD: OXFORD UNIV PRESS. ISSN 1464-3553

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Abstract

Mammalian odor reception is achieved by highly specialized olfactory sensory neurons (OSNs) located in the nasal cavity. Despite their importance for the daily survival of most mammals, the gene expression and regulatory profiles of these single neurons are poorly understood. Here, we report the isolation of individual GFP-labeled OSNs from Olfr73-GFP mice at different developmental stages followed by Next Generation Sequencing, thereby analyzing the detailed transcriptome for the first time. We characterized the repertoire of olfactory receptors (ORs) and found that in addition to the highly and predominant detectable Olfr73, 20 additional ORs were stably detectable at lower transcript levels in adult mice. Additionally, OSNs collected from mice of earlier developmental stages did not show any stable OR patterns. However, more than one predominant OR per OSN was detectable.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Scholz, Paul UNSPECIFIED UNSPECIFIED UNSPECIFIED Kalbe, Benjamin UNSPECIFIED UNSPECIFIED UNSPECIFIED Jansen, Fabian UNSPECIFIED UNSPECIFIED UNSPECIFIED Altmueller, Janine UNSPECIFIED UNSPECIFIED UNSPECIFIED Becker, Christian UNSPECIFIED UNSPECIFIED UNSPECIFIED Mohrhardt, Julia UNSPECIFIED UNSPECIFIED UNSPECIFIED Schreiner, Benjamin UNSPECIFIED UNSPECIFIED UNSPECIFIED Gisselmann, Guenter UNSPECIFIED UNSPECIFIED UNSPECIFIED Hatt, Hanns UNSPECIFIED UNSPECIFIED UNSPECIFIED Osterloh, Sabrina UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-276940
DOI:	10.1093/chemse/bjw003
Journal or Publication Title:	Chem. Senses
Volume:	41
Number:	4
Page Range:	S. 313 - 324
Date:	2016
Publisher:	OXFORD UNIV PRESS
Place of Publication:	OXFORD
ISSN:	1464-3553
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language EXPRESSION ANALYSIS; GENE; PROTEIN; RECEPTORS; SENSITIVITY; CHANNEL; SYSTEM; MUCOSA; BULB Multiple languages Behavioral Sciences; Food Science & Technology; Neurosciences; Physiology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/27694