Universität zu Köln

Brych, Annika, Mascarenhas, Judita, Jaeger, Elaine, Charkiewicz, Elzbieta, Pokorny, Richard, Boelker, Michael, Doehlemann, Gunther ORCID: 0000-0002-7353-8456 and Batschauer, Alfred (2016). White collar 1-induced photolyase expression contributes to UV-tolerance of Ustilago maydis. MicrobiologyOpen, 5 (2). S. 224 - 244. HOBOKEN: WILEY. ISSN 2045-8827

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Abstract

Ustilago maydis is a phytopathogenic fungus causing corn smut disease. It also is known for its extreme tolerance to UV- and ionizing radiation. It has not been elucidated whether light-sensing proteins, and in particular photolyases play a role in its UV-tolerance. Based on homology analysis, U. maydis has 10 genes encoding putative light-responsive proteins. Four amongst these belong to the cryptochrome/photolyase family (CPF) and one represents a white collar 1 ortholog (wco1). Deletion mutants in the predicted cyclobutane pyrimidine dimer CPD- and (6-4)-photolyase were impaired in photoreactivation. In line with this, in vitro studies with recombinant CPF proteins demonstrated binding of the catalytic FAD cofactor, its photoreduction to fully reduced FADH(-) and repair activity for cyclobutane pyrimidine dimers (CPDs) or (6-4)-photoproducts, respectively. We also investigated the role of Wco1. Strikingly, transcriptional profiling showed 61 genes differentially expressed upon blue light exposure of wild-type, but only eight genes in the wco1 mutant. These results demonstrate that Wco1 is a functional blue light photoreceptor in U. maydis regulating expression of several genes including both photolyases. Finally, we show that the wco1 mutant is less tolerant against UV-B due to its incapability to induce photolyase expression.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Brych, Annika UNSPECIFIED UNSPECIFIED UNSPECIFIED Mascarenhas, Judita UNSPECIFIED UNSPECIFIED UNSPECIFIED Jaeger, Elaine UNSPECIFIED UNSPECIFIED UNSPECIFIED Charkiewicz, Elzbieta UNSPECIFIED UNSPECIFIED UNSPECIFIED Pokorny, Richard UNSPECIFIED UNSPECIFIED UNSPECIFIED Boelker, Michael UNSPECIFIED UNSPECIFIED UNSPECIFIED Doehlemann, Gunther UNSPECIFIED orcid.org/0000-0002-7353-8456 UNSPECIFIED Batschauer, Alfred UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-279809
DOI:	10.1002/mbo3.322
Journal or Publication Title:	MicrobiologyOpen
Volume:	5
Number:	2
Page Range:	S. 224 - 244
Date:	2016
Publisher:	WILEY
Place of Publication:	HOBOKEN
ISSN:	2045-8827
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Biology > Botanical Institute
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language BLUE-LIGHT PHOTORECEPTORS; GENE REPLACEMENT MUTANTS; NEUROSPORA-CRASSA; ASPERGILLUS-NIDULANS; ARABIDOPSIS-THALIANA; SEXUAL DEVELOPMENT; DNA PHOTOLYASE; PATHOGENIC DEVELOPMENT; ESCHERICHIA-COLI; PHYTOCHROME FPHA Multiple languages Microbiology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/27980