Universität zu Köln

Michalk, Maike, Meinrath, Jeannine, Kuenstlinger, Helen, Koitzsch, Ulrike, Drebber, Uta, Merkelbach-Bruse, Sabine, Bollschweiler, Elfriede, Kloth, Michael, Hartmann, Wolfgang ORCID: 0000-0002-7609-5021, Hoelscher, Arnulf, Quaas, Alexander, Grimminger, Peter P. and Odenthal, Margarete (2016). MDM2 gene amplification in esophageal carcinoma. Oncol. Rep., 35 (4). S. 2223 - 2228. ATHENS: SPANDIDOS PUBL LTD. ISSN 1791-2431

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Abstract

Esophageal cancer (EC) is one of the most common malignancies diagnosed in the Western world with an increasing incidence noted for esophageal adenocarcinoma (EAC). Despite improvements in staging, surgical procedures and postoperative treatments, the overall survival of patients with EC remains low. Murine double minute-2 (MDM2) acts as an oncogene by inducing the degradation of the tumor-suppressor protein TP53. In order to evaluate the MDM2 gene amplification status in EAC and squamous cell carcinoma (SCC), we established a quantitative PCR (qPCR) assay, screening a total of 127 esophageal carcinoma cases for MDM2 amplification. Esophageal carcinoma cases with enhanced MDM2 gene copy numbers were further analyzed by fluorescence in situ hybridisation (FISH) and MDM2 immunostaining. Among a total of 23 specimens (18%), identified by qPCR to possess elevated MDM2 gene copy numbers, one third (6.3%) showed a cluster-like fluorescence pattern by FISH analyses and marked MDM2 protein immunostaining. MDM2 gene amplifications did not correlate with the occurrence of TP53 mutations. Due to the high therapeutic relevance of MDM2 overexpression, but the high cost of FISH, we suggest a primary screening of MDM2 copy number variations by qPCR, followed by detailed FISH analysis of the identified ECs.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Michalk, Maike UNSPECIFIED UNSPECIFIED UNSPECIFIED Meinrath, Jeannine UNSPECIFIED UNSPECIFIED UNSPECIFIED Kuenstlinger, Helen UNSPECIFIED UNSPECIFIED UNSPECIFIED Koitzsch, Ulrike UNSPECIFIED UNSPECIFIED UNSPECIFIED Drebber, Uta UNSPECIFIED UNSPECIFIED UNSPECIFIED Merkelbach-Bruse, Sabine UNSPECIFIED UNSPECIFIED UNSPECIFIED Bollschweiler, Elfriede UNSPECIFIED UNSPECIFIED UNSPECIFIED Kloth, Michael UNSPECIFIED UNSPECIFIED UNSPECIFIED Hartmann, Wolfgang UNSPECIFIED orcid.org/0000-0002-7609-5021 UNSPECIFIED Hoelscher, Arnulf UNSPECIFIED UNSPECIFIED UNSPECIFIED Quaas, Alexander UNSPECIFIED UNSPECIFIED UNSPECIFIED Grimminger, Peter P. UNSPECIFIED UNSPECIFIED UNSPECIFIED Odenthal, Margarete UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-280671
DOI:	10.3892/or.2016.4578
Journal or Publication Title:	Oncol. Rep.
Volume:	35
Number:	4
Page Range:	S. 2223 - 2228
Date:	2016
Publisher:	SPANDIDOS PUBL LTD
Place of Publication:	ATHENS
ISSN:	1791-2431
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language SQUAMOUS-CELL CARCINOMA; P53 MUTATION; CANCER; PROTEIN; ADENOCARCINOMA; EXPRESSION; SURVIVAL; TP53; POLYMORPHISMS; ACCUMULATION Multiple languages Oncology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/28067