Universität zu Köln

Jacoby, R. P., Che-Othman, M. H., Millar, A. H. and Taylor, N. L. (2016). Analysis of the sodium chloride-dependent respiratory kinetics of wheat mitochondria reveals differential effects on phosphorylating and non-phosphorylating electron transport pathways. Plant Cell Environ., 39 (4). S. 823 - 834. HOBOKEN: WILEY. ISSN 1365-3040

Full text not available from this repository.

Abstract

A number of previous studies have documented the gross response of mitochondrial respiration to salinity treatment, but it is unclear how NaCl directly affects the kinetics of plant phosphorylating and non-phosphorylating electron transport pathways. This study investigates the direct effects of NaCl upon different respiratory pathways in wheat, by measuring rates of isolated mitochondrial oxygen consumption across different substrate oxidation pathways in saline media. We also profile the abundance of respiratory proteins by using targeted selected reaction monitoring (SRM) mass spectrometry of mitochondria isolated from control and salt-treated wheat plants. We show that all pathways of electron transport were inhibited by NaCl concentrations above 400mM; however electron transfer chains showed divergent responses to NaCl concentrations between 0 and 200mM. Stimulation of oxygen consumption was measured in response to NaCl in scenarios where exogenous NADH was provided as substrate and electron flow was coupled to the generation of a proton gradient across the inner membrane. Protein abundance measurements show that several enzymes with activities less affected by NaCl are induced by salinity, whereas enzymes with activities inhibited by NaCl are depleted. These data deepen our understanding of how plant respiration responds to NaCl, offering new mechanistic explanations for the divergent salinity responses of whole-plant respiratory rate in the literature. A number of previous studies have documented the gross response of mitochondrial respiration to salinity treatment, but it is unclear how NaCl directly affects the kinetics of plant phosphorylating and non-phosphorylating electron transport pathways. This study investigates the direct effects of NaCl upon different respiratory pathways in wheat, by measuring rates of isolated mitochondrial oxygen consumption across different substrate oxidation pathways in saline media. These data deepen our understanding of how plant respiration responds to NaCl, offering new mechanistic explanations for the divergent salinity responses of whole-plant respiratory rate in the literature.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Jacoby, R. P. UNSPECIFIED UNSPECIFIED UNSPECIFIED Che-Othman, M. H. UNSPECIFIED UNSPECIFIED UNSPECIFIED Millar, A. H. UNSPECIFIED UNSPECIFIED UNSPECIFIED Taylor, N. L. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-280708
DOI:	10.1111/pce.12653
Journal or Publication Title:	Plant Cell Environ.
Volume:	39
Number:	4
Page Range:	S. 823 - 834
Date:	2016
Publisher:	WILEY
Place of Publication:	HOBOKEN
ISSN:	1365-3040
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language SALT TOLERANCE; ALTERNATIVE OXIDASE; SALINITY TOLERANCE; PLANT-MITOCHONDRIA; ION UPTAKE; PROTEIN; DEHYDROGENASE; STRESS; PROTEOMES; TOXICITY Multiple languages Plant Sciences Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/28070