Universität zu Köln

Fatima, Azra, Ivanyuk, Dina, Herms, Stefan ORCID: 0000-0002-2786-8200, Heilmann-Heimbach, Stefanie ORCID: 0000-0003-1057-465X, O'Shea, Orla, Chapman, Charlotte, Izsvak, Zsuszanna, Farr, Martin, Hescheler, Juegen and Saric, Tomo ORCID: 0000-0001-8344-1095 (2016). Generation of human induced pluripotent stem cell line from a patient with a long QT syndrome type 2. Stem Cell Res., 16 (2). S. 304 - 308. AMSTERDAM: ELSEVIER SCIENCE BV. ISSN 1876-7753

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Abstract

We report here the generation of human iPS cell line UKKi009-A from dermal fibroblasts of a patient carrying heterozygous mutation c.3035-3045delTCCCTCGATGC, p.Leu1012Pro (fs*55) in KCNH2 gene leading to long QT syndrome type 2 (LQT2). We used the Sleeping Beauty transposon-based plasmids expressing OSKM along with microRNAs 307/367 to reprogram the fibroblasts. The iPS cells possess pluripotent stem cell characteristics and differentiate to cell lineages of all three germ layers. This cell line can serve as a source for in vitro modeling of LQT2. This cell line is distributed by the European Collection of Authenticated Cell Cultures (ECACC). (C) 2016 The Authors. Published by Elsevier B.V.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Fatima, Azra UNSPECIFIED UNSPECIFIED UNSPECIFIED Ivanyuk, Dina UNSPECIFIED UNSPECIFIED UNSPECIFIED Herms, Stefan UNSPECIFIED orcid.org/0000-0002-2786-8200 UNSPECIFIED Heilmann-Heimbach, Stefanie UNSPECIFIED orcid.org/0000-0003-1057-465X UNSPECIFIED O'Shea, Orla UNSPECIFIED UNSPECIFIED UNSPECIFIED Chapman, Charlotte UNSPECIFIED UNSPECIFIED UNSPECIFIED Izsvak, Zsuszanna UNSPECIFIED UNSPECIFIED UNSPECIFIED Farr, Martin UNSPECIFIED UNSPECIFIED UNSPECIFIED Hescheler, Juegen UNSPECIFIED UNSPECIFIED UNSPECIFIED Saric, Tomo UNSPECIFIED orcid.org/0000-0001-8344-1095 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-282127
DOI:	10.1016/j.scr.2015.12.039
Journal or Publication Title:	Stem Cell Res.
Volume:	16
Number:	2
Page Range:	S. 304 - 308
Date:	2016
Publisher:	ELSEVIER SCIENCE BV
Place of Publication:	AMSTERDAM
ISSN:	1876-7753
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language Cell & Tissue Engineering; Biotechnology & Applied Microbiology; Cell Biology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/28212