Universität zu Köln

Beil-Wagner, Jane ORCID: 0000-0003-1853-8303, Doessinger, Georg, Schober, Kilian ORCID: 0000-0001-9323-9472, vom Berg, Johannes, Tresch, Achim, Grandl, Martina, Palle, Pushpalatha, Mair, Florian ORCID: 0000-0001-6732-5449, Gerhard, Markus ORCID: 0000-0001-9110-3950, Becher, Burkhard ORCID: 0000-0002-1541-7867, Busch, Dirk H. and Buch, Thorsten ORCID: 0000-0002-2236-9074 (2016). T cell-specific inactivation of mouse CD2 by CRISPR/Cas9. Sci Rep, 6. LONDON: NATURE PUBLISHING GROUP. ISSN 2045-2322

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Abstract

The CRISPR/Cas9 system can be used to mutate target sequences by introduction of double-strand breaks followed by imprecise repair. To test its use for conditional gene editing we generated mice transgenic for CD4 promoter-driven Cas9 combined with guide RNA targeting CD2. We found that within CD4(+) and CD8(+) lymphocytes from lymph nodes and spleen 1% and 0.6% were not expressing CD2, respectively. T cells lacking CD2 carryied mutations, which confirmed that Cas9 driven by cell-type specific promoters can edit genes in the mouse and may thus allow targeted studies of gene function in vivo.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Beil-Wagner, Jane UNSPECIFIED orcid.org/0000-0003-1853-8303 UNSPECIFIED Doessinger, Georg UNSPECIFIED UNSPECIFIED UNSPECIFIED Schober, Kilian UNSPECIFIED orcid.org/0000-0001-9323-9472 UNSPECIFIED vom Berg, Johannes UNSPECIFIED UNSPECIFIED UNSPECIFIED Tresch, Achim UNSPECIFIED UNSPECIFIED UNSPECIFIED Grandl, Martina UNSPECIFIED UNSPECIFIED UNSPECIFIED Palle, Pushpalatha UNSPECIFIED UNSPECIFIED UNSPECIFIED Mair, Florian UNSPECIFIED orcid.org/0000-0001-6732-5449 UNSPECIFIED Gerhard, Markus UNSPECIFIED orcid.org/0000-0001-9110-3950 UNSPECIFIED Becher, Burkhard UNSPECIFIED orcid.org/0000-0002-1541-7867 UNSPECIFIED Busch, Dirk H. UNSPECIFIED UNSPECIFIED UNSPECIFIED Buch, Thorsten UNSPECIFIED orcid.org/0000-0002-2236-9074 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-284316
DOI:	10.1038/srep21377
Journal or Publication Title:	Sci Rep
Volume:	6
Date:	2016
Publisher:	NATURE PUBLISHING GROUP
Place of Publication:	LONDON
ISSN:	2045-2322
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language ZINC-FINGER NUCLEASES; GENE; RNA; MICE; CAS9; DNA; CRISPR-CAS9; ENDONUCLEASE; SYSTEMS; RECOMBINATION Multiple languages Multidisciplinary Sciences Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/28431