Universität zu Köln

Aldeyarbi, Hebatalla M. and Karanis, Panagiotis (2016). Electron microscopic observation of the early stages of Cryptosporidium parvum asexual multiplication and development in in vitro axenic culture. Eur. J. Protistol., 52. S. 36 - 45. JENA: ELSEVIER GMBH, URBAN & FISCHER VERLAG. ISSN 1618-0429

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Abstract

The stages of Cryptosporidium parvum asexual exogenous development were investigated at high ultra-structural resolution in cell-free culture using transmission electron microscopy (TEM). Early C. parvum trophozoites were ovoid in shape, 1.07 x 1.47 mu m(2) in size, and contained a large nucleus and adjacent Golgi complex. Dividing and mature meronts containing four to eight developing merozoites, 2.34 x 2.7 mu m(2) in size, were observed within the first 24 h of cultivation. An obvious peculiarity was found within the merozoite pellicle, as it was composed of the outer plasma membrane with underlying middle and inner membrane complexes. Further novel findings were vacuolization of the meront's residuum and extension of its outer pellicle, as parasitophorous vacuole-like membranes were also evident. The asexual reproduction of C. parvum was consistent with the developmental pattern of both eimerian coccidia and Arthrogregarinida (formerly Neogregarinida). The unique cell-free development of C. parvum described here, along with the establishment of meronts and merozoite formation, is the first such evidence obtained from in vitro cell-free culture at the ultrastructural level. (C) 2015 Elsevier GmbH. All rights reserved.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Aldeyarbi, Hebatalla M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Karanis, Panagiotis UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-285422
DOI:	10.1016/j.ejop.2015.07.002
Journal or Publication Title:	Eur. J. Protistol.
Volume:	52
Page Range:	S. 36 - 45
Date:	2016
Publisher:	ELSEVIER GMBH, URBAN & FISCHER VERLAG
Place of Publication:	JENA
ISSN:	1618-0429
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language CELL-FREE CULTURE; LIFE-CYCLE STAGES; ULTRASTRUCTURAL ANALYSIS; ENDOGENOUS DEVELOPMENT; EPITHELIAL-CELLS; PARASITE; SPOROZOITE; ATTACHMENT; HOST; EXCYSTATION Multiple languages Microbiology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/28542