Universität zu Köln

Hilbrant, Maarten, Horn, Thorsten, Koelzer, Stefan and Panfilio, Kristen A. (2016). The beetle amnion and serosa functionally interact as apposed epithelia. eLife, 5. CAMBRIDGE: ELIFE SCIENCES PUBLICATIONS LTD. ISSN 2050-084X

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Abstract

Unlike passive rupture of the human chorioamnion at birth, the insect extraembryonic (EE) tissues - the amnion and serosa - actively rupture and withdraw in late embryogenesis. Withdrawal is essential for development and has been a morphogenetic puzzle. Here, we use new fluorescent transgenic lines in the beetle Tribolium castaneum to show that the EE tissues dynamically form a basal-basal epithelial bilayer, contradicting the previous hypothesis of EE intercalation. We find that the EE tissues repeatedly detach and reattach throughout development and have distinct roles. Quantitative live imaging analyses show that the amnion initiates EE rupture in a specialized anterior-ventral cap. RNAi phenotypes demonstrate that the serosa contracts autonomously. Thus, apposition in a bilayer enables the amnion as 'initiator' to coordinate with the serosa as 'driver' to achieve withdrawal. This EE strategy may reflect evolutionary changes within the holometabolous insects and serves as a model to study interactions between developing epithelia.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Hilbrant, Maarten UNSPECIFIED UNSPECIFIED UNSPECIFIED Horn, Thorsten UNSPECIFIED UNSPECIFIED UNSPECIFIED Koelzer, Stefan UNSPECIFIED UNSPECIFIED UNSPECIFIED Panfilio, Kristen A. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-286895
DOI:	10.7554/eLife.13834
Journal or Publication Title:	eLife
Volume:	5
Date:	2016
Publisher:	ELIFE SCIENCES PUBLICATIONS LTD
Place of Publication:	CAMBRIDGE
ISSN:	2050-084X
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language TRIBOLIUM EMBRYOGENESIS; BASEMENT-MEMBRANE; CELL; SPECIFICATION; MORPHOGENESIS; DYNAMICS; FEATURES; EMBRYOS; BUG Multiple languages Biology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/28689