Universität zu Köln

Hizay, Arzu ORCID: 0000-0001-5886-0714, Seitz, Mark, Grosheva, Maria ORCID: 0000-0002-5985-468X, Sinis, Nektarios, Kaya, Yasemin, Bendella, Habib, Sarikcioglu, Levent ORCID: 0000-0002-0825-6225, Dunlop, Sarah A. and Angelov, Doychin N. (2016). FGF-2 is required to prevent astrogliosis in the facial nucleus after facial nerve injury and mechanical stimulation of denervated vibrissal muscles. J. Biomed. Res., 30 (2). S. 142 - 149. NANJING: NANJING MEDICAL UNIV. ISSN 1876-4819

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Abstract

Recently, we have shown that manual stimulation of paralyzed vibrissal muscles after facial-facial anastomosis reduced the poly-innervation of neuromuscular junctions and restored vibrissal whisking. Using gene knock outs, we found a differential dependence of manual stimulation effects on growth factors. Thus, insulin-like growth factor- 1 and brain-derived neurotrophic factor are required to underpin manual stimulation-mediated improvements, whereas FGF-2 is not. The lack of dependence on FGF-2 in mediating these peripheral effects prompted us to look centrally, i. e. within the facial nucleus where increased astrogliosis after facial-facial anastomosis follows synaptic stripping. We measured the intensity of Cy3-fluorescence after immunostaining for glial fibrillary acidic protein (GFAP) as an indirect indicator of synaptic coverage of axotomized neurons in the facial nucleus of mice lacking FGF-2 (FGF-2(-/-) mice). There was no difference in GFAP-Cy3-fluorescence (pixel number, gray value range 17-103) between intact wildtype mice (2.12 +/- 0.37x10(7)) and their intact FGF-2(-/-) counterparts (2.12 +/- 0.27x10(7)) nor after facial-facial anastomosis + handling (wildtype: 4.06 +/- 0.32x10(7); FGF-2(-/-) : 4.39 +/- 0.17x10(7)). However, after facial-facial anastomosis, GFAP-Cy3-fluorescence remained elevated in FGF-2(-/-) -animals (4.54 +/- 0.12x10(7)), whereas manual stimulation reduced the intensity of GFAP-immunofluorescence in wild type mice to values that were not significantly different from intact mice (2.63 +/- 0.39x10). We conclude that FGF-2 is not required to underpin the beneficial effects of manual stimulation at the neuro-muscular junction, but it is required to minimize astrogliosis in the brainstem and, by implication, restore synaptic coverage of recovering facial motoneurons.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Hizay, Arzu UNSPECIFIED orcid.org/0000-0001-5886-0714 UNSPECIFIED Seitz, Mark UNSPECIFIED UNSPECIFIED UNSPECIFIED Grosheva, Maria UNSPECIFIED orcid.org/0000-0002-5985-468X UNSPECIFIED Sinis, Nektarios UNSPECIFIED UNSPECIFIED UNSPECIFIED Kaya, Yasemin UNSPECIFIED UNSPECIFIED UNSPECIFIED Bendella, Habib UNSPECIFIED UNSPECIFIED UNSPECIFIED Sarikcioglu, Levent UNSPECIFIED orcid.org/0000-0002-0825-6225 UNSPECIFIED Dunlop, Sarah A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Angelov, Doychin N. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-290381
DOI:	10.7555/JBR.30.20140042
Journal or Publication Title:	J. Biomed. Res.
Volume:	30
Number:	2
Page Range:	S. 142 - 149
Date:	2016
Publisher:	NANJING MEDICAL UNIV
Place of Publication:	NANJING
ISSN:	1876-4819
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language FIBRILLARY ACIDIC PROTEIN; MANUAL STIMULATION; ADULT-RATS; REACTIVE ASTROCYTES; WHISKING FUNCTION; RECOVERY; LESIONS; NEURONS; CORTEX; MICE Multiple languages Biology; Multidisciplinary Sciences Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/29038