Universität zu Köln

Weber, Stefanie, Jung, Susan and Doerfler, Walter (2016). DNA methylation and transcription in HERV (K, W, E) and LINE sequences remain unchanged upon foreign DNA insertions. Epigenomics, 8 (2). S. 157 - 166. LONDON: FUTURE MEDICINE LTD. ISSN 1750-192X

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Abstract

Aim: DNA methylation and transcriptional profiles were determined in the regulatory sequences of the human endogenous retroviral (HERV-K, -W, -E) and LINE-1.2 elements and were compared between non-transgenomic and plasmid-transgenomic cells. Methods: DNA methylation profiles in the HERV (K, W, E) and LINE sequences were determined by bisulfite genomic sequencing. The transcription of these genome segments was assessed by quantitative real-time PCR. Results: In HERV-K, HERV-W and LINE-1.2 the levels of DNA methylation ranged between 75 and 98%, while in HERV-E they were around 60%. Nevertheless, the HERV and LINE-1.2 sequences were actively transcribed. No differences were found in comparisons of HERV and LINE-1.2 CpG methylation and transcription patterns between non-transgenomic and plasmid-transgenomic HCT116 cells. Conclusion: The insertion of a 5.6 kbp plasmid into the HCT116 genome had no effect on the HERV and LINE-1.2 methylation and transcription profiles, although other parts of the HCT116 genome had shown marked changes. These repetitive sequences are transcribed, probably because the large number of HERV and LINE-1.2 elements harbor copies with non-or hypo-methylated long terminal repeat sequences.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Weber, Stefanie UNSPECIFIED UNSPECIFIED UNSPECIFIED Jung, Susan UNSPECIFIED UNSPECIFIED UNSPECIFIED Doerfler, Walter UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-291082
DOI:	10.2217/epi.15.109
Journal or Publication Title:	Epigenomics
Volume:	8
Number:	2
Page Range:	S. 157 - 166
Date:	2016
Publisher:	FUTURE MEDICINE LTD
Place of Publication:	LONDON
ISSN:	1750-192X
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language ENDOGENOUS RETROVIRUSES; CELLS; GENOME; ADENOVIRUS-TYPE-12; INTEGRATION; EXPRESSION; ELEMENTS; GENES; RNA Multiple languages Genetics & Heredity Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/29108