Universität zu Köln

Hess, Sebastian, Suthaus, Andreas and Melkonian, Michael (2016). Candidatus Finniella (Rickettsiales, Alphaproteobacteria), Novel Endosymbionts of Viridiraptorid Amoeboflagellates (Cercozoa, Rhizaria). Appl. Environ. Microbiol., 82 (2). S. 659 - 671. WASHINGTON: AMER SOC MICROBIOLOGY. ISSN 1098-5336

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Abstract

The Rickettsiales (Alphaproteobacteria) are obligate intracellular bacteria that colonize a wide range of eukaryotic hosts, including diverse metazoa and protists. Here, we characterize rickettsial endosymbionts discovered in the cytoplasm of the algivorous amoeboflagellates Viridiraptor invadens and Orciraptor agilis (Viridiraptoridae, Cercozoa, Rhizaria), supplying evidence of free-living, phagotrophic members of the Cercozoa serving as hosts for Rickettsiales. According to 16S rRNA gene phylogenies, the bacteria represent two closely related but distinct genotypes within a deep-branching rickettsial clade, which contains the genera Candidatus Odyssella, Candidatus Paracaedibacter, and Candidatus Captivus. Using the full-cycle rRNA approach, we detected the novel bacteria in four of nine viridiraptorid strains tested. Furthermore, two specific oligonucleotide probes with a single-nucleotide-difference discriminated both bacterial genotypes by fluorescence in situ hybridization (FISH). We establish the candidate species Candidatus Finniella inopinata (found in Viridiraptor invadens) and Candidatus Finniella lucida (found in Orciraptor agilis) for the novel bacteria and propose a new, provisional family of Rickettsiales, Candidatus Paracaedibacteraceae.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Hess, Sebastian UNSPECIFIED UNSPECIFIED UNSPECIFIED Suthaus, Andreas UNSPECIFIED UNSPECIFIED UNSPECIFIED Melkonian, Michael UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-291313
DOI:	10.1128/AEM.02680-15
Journal or Publication Title:	Appl. Environ. Microbiol.
Volume:	82
Number:	2
Page Range:	S. 659 - 671
Date:	2016
Publisher:	AMER SOC MICROBIOLOGY
Place of Publication:	WASHINGTON
ISSN:	1098-5336
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language TARGETED OLIGONUCLEOTIDE PROBES; TICK IXODES-RICINUS; BACTERIAL ENDOSYMBIONTS; SEQUENCE ALIGNMENT; ACANTHAMOEBA SPP.; INTRACELLULAR BACTERIA; ONLINE RESOURCE; GEN. NOV; SYMBIONT; ULTRASTRUCTURE Multiple languages Biotechnology & Applied Microbiology; Microbiology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/29131