Prax, Marcel, Spindler-Raffel, Eva, McDonald, Carl Peter, Bearne, Jennifer, Satake, Masahiro, Kozakai, Moe, Rojo, Julieta, Hanschmann, Kay-Martin O., Lambrecht, Bernd, Grundmann, Udo, O'Flaherty, Niamh, Klimek, Agata, Bekeredjian-Ding, Isabelle, Gathof, Birgit S., Stormer, Melanie, Suesner, Susanne, Renke, Claudia, Lee, Cheuk-kwong, Knabbe, Cornelius, Vollmer, Tanja, Keil, Shawn D., Shipps, Marley E., Wagner, Stephen J., Jentsch, Ute, Mpumlwana, Xoliswa, Cloutier, Marc, Bringmann, Peter, Lu, Thea, Ramirez-Arcos, Sandra, Kou, Yuntong. and Krut, Oleg . Establishment of Transfusion-Relevant Bacteria Reference Strains for Red Blood Cells. Vox Sang.. HOBOKEN: WILEY. ISSN 1423-0410

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Abstract

Background and objectives Red blood cell concentrates (RBCC) are susceptible to bacterial contamination despite cold storage. A reliable evaluation of strategies to minimize the risk of RBCC-associated bacterial transmission requires the use of suitable reference bacteria. Already existing Transfusion-Relevant Bacteria Reference Strains (TRBRS) for platelet concentrates fail to grow in RBCC. Consequently, the ISBT TTID, Working Party, Bacterial Subgroup, conducted an international study on TRBRS for RBCC. Materials and methods Six bacterial strains (Listeria monocytogenes PEI-A-199, Serratia liquefaciens PEI-A-184, Serratia marcescens PEI-B-P-56, Pseudomonas fluorescens PEI-B-P-77, Yersinia enterocolitica PEI-A-105, Yersinia enterocolitica PEI-A-176) were distributed to 15 laboratories worldwide for enumeration, identification, and determination of growth kinetics in RBCC at days 7, 14, 21, 28, 35 and 42 of storage after low-count spiking (10-25 CFU/RBCC). Results Bacterial proliferation in RBCC was obtained for most strains, except for S. marcescens, which grew only at 4 of 15 laboratories. S. liquefaciens, S. marcescens, P. fluorescens and the two Y. enterocolitica strains reached the stationary phase between days 14 and 21 of RBCC storage with a bacterial concentration of approximately 10(9) CFU/ml. L. monocytogenes displayed slower growth kinetics reaching 10(6)-10(7) CFU/ml after 42 days. Conclusion The results illustrate the importance of conducting comprehensive studies to establish well-characterized reference strains, which can be a tool to assess strategies and methods used to ameliorate blood safety. The WHO Expert Committee on Biological Standardization adopted the five successful strains as official RBCC reference strains. Our study also highlights the relevance of visual inspection to interdict contaminated RBC units.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Prax, MarcelUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Spindler-Raffel, EvaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
McDonald, Carl PeterUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Bearne, JenniferUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Satake, MasahiroUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Kozakai, MoeUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Rojo, JulietaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Hanschmann, Kay-Martin O.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Lambrecht, BerndUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Grundmann, UdoUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
O'Flaherty, NiamhUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Klimek, AgataUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Bekeredjian-Ding, IsabelleUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Gathof, Birgit S.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Stormer, MelanieUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Suesner, SusanneUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Renke, ClaudiaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Lee, Cheuk-kwongUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Knabbe, CorneliusUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Vollmer, TanjaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Keil, Shawn D.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Shipps, Marley E.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Wagner, Stephen J.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Jentsch, UteUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Mpumlwana, XoliswaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Cloutier, MarcUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Bringmann, PeterUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Lu, TheaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Ramirez-Arcos, SandraUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Kou, Yuntong.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Krut, OlegUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-307668
DOI: 10.1111/vox.13057
Journal or Publication Title: Vox Sang.
Publisher: WILEY
Place of Publication: HOBOKEN
ISSN: 1423-0410
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
YERSINIA-ENTEROCOLITICA; INTERNATIONAL REPOSITORY; QUALITY; COMPLEX; SEPSIS; GROWTH; RISKMultiple languages
HematologyMultiple languages
URI: http://kups.ub.uni-koeln.de/id/eprint/30766

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