Mahdi, Lisa K., Huang, Menghang, Zhang, Xiaoxiao, Nakano, Ryohei Thomas, Kopp, Leila Brule, Saur, Isabel M. L., Jacob, Florence, Kovacova, Viera, Lapin, Dmitry, Parker, Jane E., Murphy, James M., Hofmann, Kay, Schulze-Lefert, Paul, Chai, Jijie and Maekawa, Takaki (2020). Discovery of a Family of Mixed Lineage Kinase Domain-like Proteins in Plants and Their Role in Innate Immune Signaling. Cell Host Microbe, 28 (6). S. 813 - 831. CAMBRIDGE: CELL PRESS. ISSN 1934-6069

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Abstract

HeLo domain-containing mixed lineage kinase domain-like protein (MLKL), a pseudokinase, mediates necroptotic cell death in animals. Here, we report the discovery of a conserved protein family across seed plants that structurally resembles vertebrate MLKL. The Arabidopsis genome encodes three MLKLs (AtMLKLs) with overlapping functions in disease resistance mediated by Toll-interleukin 1-receptor domain intracellular immune receptors (TNLs). The HeLo domain of AtMLKLs confers cell death activity but is dispensable for immunity. Cryo-EM structures reveal a tetrameric configuration, in which the HeLo domain is buried, suggestive of an auto-repressed complex. The mobility of AtMLKL1 along microtubules is reduced by chitin, a fungal immunity-triggering molecule. An AtMLKL1 phosphomimetic variant exhibiting reduced mobility enhances immunity. Coupled with the predicted presence of HeLo domains in plant helper NLRs, our data reveal the importance of HeLo domain proteins for TNL-dependent immunity and argue for a cell death-independent immune mechanism mediated by MLKLs.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Mahdi, Lisa K.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Huang, MenghangUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Zhang, XiaoxiaoUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Nakano, Ryohei ThomasUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Kopp, Leila BruleUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Saur, Isabel M. L.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Jacob, FlorenceUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Kovacova, VieraUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Lapin, DmitryUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Parker, Jane E.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Murphy, James M.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Hofmann, KayUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Schulze-Lefert, PaulUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Chai, JijieUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Maekawa, TakakiUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-308407
DOI: 10.1016/j.chom.2020.08.012
Journal or Publication Title: Cell Host Microbe
Volume: 28
Number: 6
Page Range: S. 813 - 831
Date: 2020
Publisher: CELL PRESS
Place of Publication: CAMBRIDGE
ISSN: 1934-6069
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
PROGRAMMED CELL-DEATH; PATTERN-RECOGNITION RECEPTORS; DISEASE RESISTANCE GENES; ARABIDOPSIS-THALIANA; TIR DOMAINS; GABI-KAT; MEMBRANE; NECROPTOSIS; SPECIFICITY; DOWNSTREAMMultiple languages
Microbiology; Parasitology; VirologyMultiple languages
URI: http://kups.ub.uni-koeln.de/id/eprint/30840

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