Renner, Alexander M., Ilyas, Shaista, Schlosser, Hans A., Szymura, Annika, Roitsch, Stefan, Wennhold, Kerstin and Mathur, Sanjay (2020). Receptor-Mediated In Vivo Targeting of Breast Cancer Cells with 17 alpha-Ethynylestradiol-Conjugated Silica-Coated Gold Nanoparticles. Langmuir, 36 (48). S. 14819 - 14829. WASHINGTON: AMER CHEMICAL SOC. ISSN 0743-7463

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Abstract

Efficient therapies for breast cancer remain elusive because of the lack of strategies for targeted transport and receptor-mediated uptake of synthetic drug molecules by cancer cells. Conjugation of nanoparticles (NPs) with active targeting ligands enabling selective molecular recognition of antigens expressed on the surface of cancer cells is promising for localization and treatment of malignant cells. In this study, covalent attachment of synthetic estrogen 17 alpha-ethynylestradiol on the silica (SiO2) shell of silica-gold NPs (SiO2@Au) was undertaken to improve the cancer-targeting ability of the nano-biotags. Chemical and structural analysis of the bioconjugates examined in solution (UV-vis and xi-potential) and solid state (Fourier transform infrared spectroscopy, X-ray diffractometry, and transmission electron microscopy) confirmed the identity of the carrier particles and surface-bound ligands. The mesoporous silica shell served as a reservoir for anticancer drugs (doxorubicin and quercetin) and to facilitate covalent attachment of receptor molecules by click chemistry protocols. The chemoselective recognition between the nanoconjugates and cell membranes was successfully demonstrated by the accumulation of nanoprobes in the tumor tissue of mice with subcutaneous breast cancer, whereas healthy cells were unaffected. The drug release studies showed sustained release kinetics over several weeks. These findings elaborate the exceptional selectivity and potential of estrogen-coated nano-biolabels in efficient diagnosis and detection of breast cancer cells.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Renner, Alexander M.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Ilyas, ShaistaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Schlosser, Hans A.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Szymura, AnnikaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Roitsch, StefanUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Wennhold, KerstinUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Mathur, SanjayUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-308505
DOI: 10.1021/acs.langmuir.0c02820
Journal or Publication Title: Langmuir
Volume: 36
Number: 48
Page Range: S. 14819 - 14829
Date: 2020
Publisher: AMER CHEMICAL SOC
Place of Publication: WASHINGTON
ISSN: 0743-7463
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
MESOPOROUS SILICA; NANORODS; DRUG; CONJUGATION; APOPTOSISMultiple languages
Chemistry, Multidisciplinary; Chemistry, Physical; Materials Science, MultidisciplinaryMultiple languages
URI: http://kups.ub.uni-koeln.de/id/eprint/30850

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