Universität zu Köln

Ma, Shoucai, Lapin, Dmitry, Liu, Li, Sun, Yue, Song, Wen, Zhang, Xiaoxiao, Logemann, Elke, Yu, Dongli, Wang, Jia, Jirschitzka, Jan, Han, Zhifu, Schulze-Lefert, Paul, Parker, Jane E. and Chai, Jijie (2020). Direct pathogen-induced assembly of an NLR immune receptor complex to form a holoenzyme. Science, 370 (6521). S. 1184 - 1195. WASHINGTON: AMER ASSOC ADVANCEMENT SCIENCE. ISSN 1095-9203

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Abstract

Direct or indirect recognition of pathogen-derived effectors by plant nucleotide-binding leucine-rich repeat (LRR) receptors (NLRs) initiates innate immune responses. The Hyaloperonospora arabidopsidis effector ATR1 activates the N-terminal Toll-interleukin-1 receptor (TIR) domain of Arabidopsis NLR RPP1. We report a cryo-electron microscopy structure of RPP1 bound by ATR1. The structure reveals a C-terminal jelly roll/ Ig-like domain (C-JID) for specific ATR1 recognition. Biochemical and functional analyses show that ATR1 binds to the C-JID and the LRRs to induce an RPP1 tetrameric assembly required for nicotinamide adenine dinucleotide hydrolase (NADase) activity. RPP1 tetramerization creates two potential active sites, each formed by an asymmetric TIR homodimer. Our data define the mechanism of direct effector recognition by a plant NLR leading to formation of a signaling-active holoenzyme.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Ma, Shoucai UNSPECIFIED UNSPECIFIED UNSPECIFIED Lapin, Dmitry UNSPECIFIED UNSPECIFIED UNSPECIFIED Liu, Li UNSPECIFIED UNSPECIFIED UNSPECIFIED Sun, Yue UNSPECIFIED UNSPECIFIED UNSPECIFIED Song, Wen UNSPECIFIED UNSPECIFIED UNSPECIFIED Zhang, Xiaoxiao UNSPECIFIED UNSPECIFIED UNSPECIFIED Logemann, Elke UNSPECIFIED UNSPECIFIED UNSPECIFIED Yu, Dongli UNSPECIFIED UNSPECIFIED UNSPECIFIED Wang, Jia UNSPECIFIED UNSPECIFIED UNSPECIFIED Jirschitzka, Jan UNSPECIFIED UNSPECIFIED UNSPECIFIED Han, Zhifu UNSPECIFIED UNSPECIFIED UNSPECIFIED Schulze-Lefert, Paul UNSPECIFIED UNSPECIFIED UNSPECIFIED Parker, Jane E. UNSPECIFIED UNSPECIFIED UNSPECIFIED Chai, Jijie UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-308659
DOI:	10.1126/science.abe3069
Journal or Publication Title:	Science
Volume:	370
Number:	6521
Page Range:	S. 1184 - 1195
Date:	2020
Publisher:	AMER ASSOC ADVANCEMENT SCIENCE
Place of Publication:	WASHINGTON
ISSN:	1095-9203
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Chemistry > Institute of Biochemistry
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language Multidisciplinary Sciences Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/30865