Kaw, Sheetal, Ananth, Swetha, Tsopoulidis, Nikolaos, Morath, Katharina, Coban, Bahar M., Hohenberger, Ralph, Bulut, Olcay C., Klein, Florian, Stolp, Bettina and Fackler, Oliver T. . Expression of HIV-1 pathogenesis factor NEF in CD4 T cells impairs antigen-specific B-cell function. Embo J.. HOBOKEN: WILEY. ISSN 1460-2075
Full text not available from this repository.Abstract
Failures to produce neutralizing antibodies upon HIV-1 infection result in part from B-cell dysfunction due to unspecific B-cell activation. How HIV-1 affects antigen-specific B-cell functions remains elusive. Using an adoptive transfer mouse model and ex vivo HIV infection of human tonsil tissue, we found that expression of the HIV-1 pathogenesis factor NEF in CD4 T cells undermines their helper function and impairs cognate B-cell functions including mounting of efficient specific IgG responses. NEF interfered with T cell help via a specific protein interaction motif that prevents polarized cytokine secretion at the T-cell-B-cell immune synapse. This interference reduced B-cell activation and proliferation and thus disrupted germinal center formation and affinity maturation. These results identify NEF as a key component for HIV-mediated dysfunction of antigen-specific B cells. Therapeutic targeting of the identified molecular surface in NEF will facilitate host control of HIV infection.
Item Type: | Journal Article | ||||||||||||||||||||||||||||||||||||||||||||
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URN: | urn:nbn:de:hbz:38-312194 | ||||||||||||||||||||||||||||||||||||||||||||
DOI: | 10.15252/embj.2020105594 | ||||||||||||||||||||||||||||||||||||||||||||
Journal or Publication Title: | Embo J. | ||||||||||||||||||||||||||||||||||||||||||||
Publisher: | WILEY | ||||||||||||||||||||||||||||||||||||||||||||
Place of Publication: | HOBOKEN | ||||||||||||||||||||||||||||||||||||||||||||
ISSN: | 1460-2075 | ||||||||||||||||||||||||||||||||||||||||||||
Language: | English | ||||||||||||||||||||||||||||||||||||||||||||
Faculty: | Unspecified | ||||||||||||||||||||||||||||||||||||||||||||
Divisions: | Unspecified | ||||||||||||||||||||||||||||||||||||||||||||
Subjects: | no entry | ||||||||||||||||||||||||||||||||||||||||||||
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URI: | http://kups.ub.uni-koeln.de/id/eprint/31219 |
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