Xanthopoulou, Kyriaki ORCID: 0000-0001-8591-8184, Carattoli, Alessandra, Wille, Julia, Biehl, Lena M., Rohde, Holger, Farowski, Fedja, Krut, Oleg, Villa, Laura, Feudi, Claudia, Seifert, Harald and Higgins, Paul G. ORCID: 0000-0001-8677-9454 (2020). Antibiotic Resistance and Mobile Genetic Elements in Extensively Drug-Resistant Klebsiella pneumoniae Sequence Type 147 Recovered from Germany. Antibiotics-Basel, 9 (10). BASEL: MDPI. ISSN 2079-6382

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Abstract

Mobile genetic elements (MGEs), especially multidrug-resistance plasmids, are major vehicles for the dissemination of antimicrobial resistance determinants. Herein, we analyse the MGEs in three extensively drug-resistant (XDR) Klebsiella pneumoniae isolates from Germany. Whole genome sequencing (WGS) is performed using Illumina and MinION platforms followed by core-genome multi-locus sequence typing (MLST). The plasmid content is analysed by conjugation, S1-pulsed-field gel electrophoresis (S1-PFGE) and Southern blot experiments. The K. pneumoniae isolates belong to the international high-risk clone ST147 and form a cluster of closely related isolates. They harbour the bla(OXA-181) carbapenemase on a ColKP3 plasmid, and 12 antibiotic resistance determinants on an multidrug-resistant (MDR) IncR plasmid with a recombinogenic nature and encoding a large number of insertion elements. The IncR plasmids within the three isolates share a high degree of homology, but present also genetic variations, such as inversion or deletion of genetic regions in close proximity to MGEs. In addition, six plasmids not harbouring any antibiotic resistance determinants are present in each isolate. Our study indicates that genetic variations can be observed within a cluster of closely related isolates, due to the dynamic nature of MGEs. The mobilome of the K. pneumoniae isolates combined with the emergence of the XDR ST147 high-risk clone have the potential to become a major challenge for global healthcare.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Xanthopoulou, KyriakiUNSPECIFIEDorcid.org/0000-0001-8591-8184UNSPECIFIED
Carattoli, AlessandraUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Wille, JuliaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Biehl, Lena M.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Rohde, HolgerUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Farowski, FedjaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Krut, OlegUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Villa, LauraUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Feudi, ClaudiaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Seifert, HaraldUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Higgins, Paul G.UNSPECIFIEDorcid.org/0000-0001-8677-9454UNSPECIFIED
URN: urn:nbn:de:hbz:38-316513
DOI: 10.3390/antibiotics9100675
Journal or Publication Title: Antibiotics-Basel
Volume: 9
Number: 10
Date: 2020
Publisher: MDPI
Place of Publication: BASEL
ISSN: 2079-6382
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
D BETA-LACTAMASE; ANTIMICROBIAL RESISTANCE; PLASMID; CARBAPENEMASE; IDENTIFICATION; EPIDEMIOLOGY; OUTBREAK; STRAINS; SPREAD; QNRS1Multiple languages
Infectious Diseases; Pharmacology & PharmacyMultiple languages
URI: http://kups.ub.uni-koeln.de/id/eprint/31651

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