Universität zu Köln

Hieggelke, Lena and Schultheis, Anne M. (2020). Application of FISH in the diagnosis of lung cancer. Pathologe, 41 (6). S. 582 - 589. HEIDELBERG: SPRINGER HEIDELBERG. ISSN 1432-1963

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Abstract

Rapid advancements in the area of lung cancer therapy were determined by the discovery of molecular markers and the possibility of their therapeutic exploitation. Today's lung cancer diagnosis places high demands on pathologists. In the majority of cases, small tissue samples must suffice for diagnosis and testing of all relevant biomarkers. The minimum panel required for advanced non-small-cell lung carcinoma (NSCLC) with nonsquamous histology includes testing ofEGFR,BRAF,ALK,ROS1, and PD-L1-expression. So far, only PD-L1-IHC (immunohistochemistry, IHC) is required for squamous cell carcinoma. If possible, newer biomarkers such asRET,MET,HER2,NTRK,andKRASshould be integrated in test panels. Fluorescence in situ hybridization (FISH) is a well-established molecular method for the detection of chromosomal aberrations, such asALK-,ROS1-,andRET- translocations and amplifications, such asHer2/neuorMET. The relevance ofMET-FISH for the detection of amplifications in the first-line setting is controversial, but of high importance in the recurrent setting. All equivocal or discrepant results should be validated using orthogonal methods. IHC is a suitable, thoroughly validated method forALKandROS1aberration detection with the advantage of quick and cost-efficient test results and tissue conservation. All other translocations, or discrepancy between IHC and FISH, require a sequencing-based confirmation procedure. The low frequency ofNTRKfusions, and high sensitivity of NTRK-IHC, suggest using IHC as a prescreening tool with subsequent sequencing-based analysis for IHC positive cases.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Hieggelke, Lena UNSPECIFIED UNSPECIFIED UNSPECIFIED Schultheis, Anne M. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-318185
DOI:	10.1007/s00292-020-00831-7
Journal or Publication Title:	Pathologe
Volume:	41
Number:	6
Page Range:	S. 582 - 589
Date:	2020
Publisher:	SPRINGER HEIDELBERG
Place of Publication:	HEIDELBERG
ISSN:	1432-1963
Language:	German
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language MET AMPLIFICATION; TARGETING MET; MUTATIONS; REARRANGEMENT; INHIBITION; RESISTANCE; PATTERN; GENE; RET Multiple languages Pathology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/31818