Universität zu Köln

Linnemannstoens, Karen, Witte, Leonie, Karuna, Pradhipa M., Kittel, Jeanette Clarissa, Danieli, Adi, Mueller, Denise, Nitsch, Lena, Honemann-Capito, Mona, Grawe, Ferdinand, Wodarz, Andreas ORCID: 0000-0002-0007-6289 and Gross, Julia Christina (2020). Ykt6-dependent endosomal recycling is required for Wnt secretion in the Drosophila wing epithelium. Development, 147 (15). CAMBRIDGE: COMPANY BIOLOGISTS LTD. ISSN 1477-9129

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Abstract

Morphogens are important signalling molecules for tissue development and their secretion requires tight regulation. In the wing imaginal disc of flies, the morphogen Wnt/Wingless is apically presented by the secreting cell and re-internalized before final long-range secretion. Why Wnt molecules undergo these trafficking steps and the nature of the regulatory control within the endosomal compartment remain unclear. Here, we have investigated how Wnts are sorted at the level of endosomes by the versatile v-SNARE Ykt6. Using in vivo genetics, proximity-dependent proteomics and in vitro biochemical analyses, we show that most Ykt6 is present in the cytosol, but can be recruited to de-acidified compartments and recycle Wnts to the plasma membrane via Rab4-positive recycling endosomes. Thus, we propose a molecular mechanism by which producing cells integrate and leverage endocytosis and recycling via Ykt6 to coordinate extracellular Wnt levels.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Linnemannstoens, Karen UNSPECIFIED UNSPECIFIED UNSPECIFIED Witte, Leonie UNSPECIFIED UNSPECIFIED UNSPECIFIED Karuna, Pradhipa M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Kittel, Jeanette Clarissa UNSPECIFIED UNSPECIFIED UNSPECIFIED Danieli, Adi UNSPECIFIED UNSPECIFIED UNSPECIFIED Mueller, Denise UNSPECIFIED UNSPECIFIED UNSPECIFIED Nitsch, Lena UNSPECIFIED UNSPECIFIED UNSPECIFIED Honemann-Capito, Mona UNSPECIFIED UNSPECIFIED UNSPECIFIED Grawe, Ferdinand UNSPECIFIED UNSPECIFIED UNSPECIFIED Wodarz, Andreas UNSPECIFIED orcid.org/0000-0002-0007-6289 UNSPECIFIED Gross, Julia Christina UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-324287
DOI:	10.1242/dev.185421
Journal or Publication Title:	Development
Volume:	147
Number:	15
Date:	2020
Publisher:	COMPANY BIOLOGISTS LTD
Place of Publication:	CAMBRIDGE
ISSN:	1477-9129
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language SNARE COMPLEX; P24 PROTEINS; ENDOCYTOSIS; GENE; RECEPTOR; PROMOTES; HEDGEHOG; WNTLESS; PATHWAY; SCREEN Multiple languages Developmental Biology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/32428