Universität zu Köln

Witte, Leonie, Linnemannstoens, Karen, Schmidt, Kevin, Honemann-Capito, Mona, Grawe, Ferdinand, Wodarz, Andreas ORCID: 0000-0002-0007-6289 and Gross, Julia Christina (2020). The kinesin motor Klp98A mediates apical to basal Wg transport. Development, 147 (15). CAMBRIDGE: COMPANY BIOLOGISTS LTD. ISSN 1477-9129

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Abstract

Development and tissue homeostasis rely on the tight regulation of morphogen secretion. In the Drosophila wing imaginal disc epithelium, Wg secretion for long-range signal transduction occurs after apical Wg entry into the endosomal system, followed by secretory endosomal transport. Although Wg release appears to occur from the apical and basal cell sides, its exact post-endocytic fate and the functional relevance of polarized endosomal Wg trafficking are poorly understood. Here, we identify the kinesin-3 family member Klp98A as the master regulator of intracellular Wg transport after apical endocytosis. In the absence of Klp98A, functional mature endosomes accumulate in the apical cytosol, and endosome transport to the basal cytosol is perturbed. Despite the resulting Wg mislocalization, Wg signal transduction occurs normally. We conclude that transcytosis-independent routes for Wg trafficking exist and demonstrate that Wg can be recycled apically via Rab4-recycling endosomes in the absence of Klp98A.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Witte, Leonie UNSPECIFIED UNSPECIFIED UNSPECIFIED Linnemannstoens, Karen UNSPECIFIED UNSPECIFIED UNSPECIFIED Schmidt, Kevin UNSPECIFIED UNSPECIFIED UNSPECIFIED Honemann-Capito, Mona UNSPECIFIED UNSPECIFIED UNSPECIFIED Grawe, Ferdinand UNSPECIFIED UNSPECIFIED UNSPECIFIED Wodarz, Andreas UNSPECIFIED orcid.org/0000-0002-0007-6289 UNSPECIFIED Gross, Julia Christina UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-324292
DOI:	10.1242/dev.186833
Journal or Publication Title:	Development
Volume:	147
Number:	15
Date:	2020
Publisher:	COMPANY BIOLOGISTS LTD
Place of Publication:	CAMBRIDGE
ISSN:	1477-9129
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language ENDOSOMAL TRAFFICKING; WINGLESS; WNT; SECRETION; HEDGEHOG; RECEPTOR; PATHWAY; TRANSCYTOSIS; ENDOCYTOSIS; PROTEINS Multiple languages Developmental Biology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/32429