Universität zu Köln

Heidepriem, Jasmin ORCID: 0000-0002-1537-6487, Kraehling, Verena, Dahlke, Christine, Wolf, Timo, Klein, Florian, Addo, Marylyn M., Becker, Stephan and Loeffler, Felix F. (2020). Epitopes of Naturally Acquired and Vaccine-Induced Anti-Ebola Virus Glycoprotein Antibodies in Single Amino Acid Resolution. Biotechnol. J., 15 (9). WEINHEIM: WILEY-V C H VERLAG GMBH. ISSN 1860-7314

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Abstract

The Ebola virus (EBOV) can cause severe infections in humans, leading to a fatal outcome in a high percentage of cases. Neutralizing antibodies against the EBOV surface glycoprotein (GP) can prevent infections, demonstrating a straightforward way for an efficient vaccination strategy. Meanwhile, many different anti-EBOV antibodies have been identified, whereas the exact binding epitopes are often unknown. Here, the analysis of serum samples from an EBOV vaccine trial with the recombinant vesicular stomatitis virus-Zaire ebolavirus (rVSV-ZEBOV) and an Ebola virus disease survivor, using high-density peptide arrays, is presented. In this proof-of-principle study, distinct IgG and IgM antibodies binding to different epitopes of EBOV GP is detected: By mapping the whole GP as overlapping peptide fragments, new epitopes and confirmed epitopes from the literature are found. Furthermore, the highly selective binding epitope of a neutralizing monoclonal anti-EBOV GP antibody could be validated. This shows that peptide arrays can be a valuable tool to study the humoral immune response to vaccines in patients and to support Ebola vaccine development.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Heidepriem, Jasmin UNSPECIFIED orcid.org/0000-0002-1537-6487 UNSPECIFIED Kraehling, Verena UNSPECIFIED UNSPECIFIED UNSPECIFIED Dahlke, Christine UNSPECIFIED UNSPECIFIED UNSPECIFIED Wolf, Timo UNSPECIFIED UNSPECIFIED UNSPECIFIED Klein, Florian UNSPECIFIED UNSPECIFIED UNSPECIFIED Addo, Marylyn M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Becker, Stephan UNSPECIFIED UNSPECIFIED UNSPECIFIED Loeffler, Felix F. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-330422
DOI:	10.1002/biot.202000069
Journal or Publication Title:	Biotechnol. J.
Volume:	15
Number:	9
Date:	2020
Publisher:	WILEY-V C H VERLAG GMBH
Place of Publication:	WEINHEIM
ISSN:	1860-7314
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language MONOCLONAL-ANTIBODIES; VIRION GLYCOPROTEINS; PEPTIDE ARRAYS; INFECTION Multiple languages Biochemical Research Methods; Biotechnology & Applied Microbiology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/33042