Universität zu Köln

Diestelkoetter-Bachert, Petra, Beck, Rainer, Reckmann, Inge, Hellwig, Andrea, Garcia-Saez, Ana, Zelman-Hopf, Monika, Hanke, Anton, Alves, Ariane Nunes ORCID: 0000-0002-5488-4732, Wade, Rebecca C., Mayer, Matthias P. and Wieland, Felix (2020). Structural characterization of an Arf dimer interface: molecular mechanism of Arf-dependent membrane scission. FEBS Lett., 594 (14). S. 2240 - 2254. HOBOKEN: WILEY. ISSN 1873-3468

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Abstract

Dimerization of the small GTPase Arf is prerequisite for the scission of COPI-coated transport vesicles. Here, we quantify the monomer/dimer equilibrium of Arf within the membrane and show that after membrane scission, Arf dimers are restricted to donor membranes. By hydrogen exchange mass spectrometry, we define the interface of activated dimeric Arf within its switch II region. Single amino acid exchanges in this region reduce the propensity of Arf to dimerize. We suggest a mechanism of membrane scission by which the dimeric form of Arf is segregated to the donor membrane. Our data are consistent with the previously reported absence of dimerized Arf in COPI vesicles and could explain the presence of one single scar-like noncoated region in each COPI vesicle.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Diestelkoetter-Bachert, Petra UNSPECIFIED UNSPECIFIED UNSPECIFIED Beck, Rainer UNSPECIFIED UNSPECIFIED UNSPECIFIED Reckmann, Inge UNSPECIFIED UNSPECIFIED UNSPECIFIED Hellwig, Andrea UNSPECIFIED UNSPECIFIED UNSPECIFIED Garcia-Saez, Ana UNSPECIFIED UNSPECIFIED UNSPECIFIED Zelman-Hopf, Monika UNSPECIFIED UNSPECIFIED UNSPECIFIED Hanke, Anton UNSPECIFIED UNSPECIFIED UNSPECIFIED Alves, Ariane Nunes UNSPECIFIED orcid.org/0000-0002-5488-4732 UNSPECIFIED Wade, Rebecca C. UNSPECIFIED UNSPECIFIED UNSPECIFIED Mayer, Matthias P. UNSPECIFIED UNSPECIFIED UNSPECIFIED Wieland, Felix UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-332537
DOI:	10.1002/1873-3468.13808
Journal or Publication Title:	FEBS Lett.
Volume:	594
Number:	14
Page Range:	S. 2240 - 2254
Date:	2020
Publisher:	WILEY
Place of Publication:	HOBOKEN
ISSN:	1873-3468
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language ADP-RIBOSYLATION FACTOR-1; COPI-COATED VESICLES; GUANINE-NUCLEOTIDE EXCHANGE; WEB SERVER; GTP; COATOMER; PROTEINS; CURVATURE; FISSION; COMPLEX Multiple languages Biochemistry & Molecular Biology; Biophysics; Cell Biology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/33253