Universität zu Köln

Solagna, Francesca, Nogara, Leonardo, Dyar, Kenneth A., Greulich, Franziska, Mir, Ashfaq A., Turk, Clara, Bock, Theresa, Geremia, Alessia, Baraldo, Martina, Sartori, Roberta ORCID: 0000-0002-2763-9838, Farup, Jean ORCID: 0000-0002-7579-6512, Uhlenhaut, Henriette ORCID: 0000-0002-4220-4779, Vissing, Kristian, Krueger, Marcus ORCID: 0000-0003-2008-4582 and Blaauw, Bert (2020). Exercise-dependent increases in protein synthesis are accompanied by chromatin modifications and increased MRTF-SRF signalling. Acta Physiol., 230 (1). HOBOKEN: WILEY. ISSN 1748-1716

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Abstract

Aim Resistance exercise increases muscle mass over time. However, the early signalling events leading to muscle growth are not yet well-defined. Here, we aim to identify new signalling pathways important for muscle remodelling after exercise. Methods We performed a phosphoproteomics screen after a single bout of exercise in mice. As an exercise model we used unilateral electrical stimulation in vivo and treadmill running. We analysed muscle biopsies from human subjects to verify if our findings in murine muscle also translate to exercise in humans. Results We identified a new phosphorylation site on Myocardin-Related Transcription Factor B (MRTF-B), a co-activator of serum response factor (SRF). Phosphorylation of MRTF-B is required for its nuclear translocation after exercise and is accompanied by the transcription of the SRF target gene Fos. In addition, high-intensity exercise also remodels chromatin at specific SRF target gene loci through the phosphorylation of histone 3 on serine 10 in myonuclei of both mice and humans. Ablation of the MAP kinase member MSK1/2 is sufficient to prevent this histone phosphorylation, reduce induction of SRF-target genes, and prevent increases in protein synthesis after exercise. Conclusion Our results identify a new exercise signalling fingerprint in vivo, instrumental for exercise-induced protein synthesis and potentially muscle growth.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Solagna, Francesca UNSPECIFIED UNSPECIFIED UNSPECIFIED Nogara, Leonardo UNSPECIFIED UNSPECIFIED UNSPECIFIED Dyar, Kenneth A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Greulich, Franziska UNSPECIFIED UNSPECIFIED UNSPECIFIED Mir, Ashfaq A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Turk, Clara UNSPECIFIED UNSPECIFIED UNSPECIFIED Bock, Theresa UNSPECIFIED UNSPECIFIED UNSPECIFIED Geremia, Alessia UNSPECIFIED UNSPECIFIED UNSPECIFIED Baraldo, Martina UNSPECIFIED UNSPECIFIED UNSPECIFIED Sartori, Roberta UNSPECIFIED orcid.org/0000-0002-2763-9838 UNSPECIFIED Farup, Jean UNSPECIFIED orcid.org/0000-0002-7579-6512 UNSPECIFIED Uhlenhaut, Henriette UNSPECIFIED orcid.org/0000-0002-4220-4779 UNSPECIFIED Vissing, Kristian UNSPECIFIED UNSPECIFIED UNSPECIFIED Krueger, Marcus UNSPECIFIED orcid.org/0000-0003-2008-4582 UNSPECIFIED Blaauw, Bert UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-332578
DOI:	10.1111/apha.13496
Journal or Publication Title:	Acta Physiol.
Volume:	230
Number:	1
Date:	2020
Publisher:	WILEY
Place of Publication:	HOBOKEN
ISSN:	1748-1716
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Biology > Institute for Genetics
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language HUMAN SKELETAL-MUSCLE; ACTIN DYNAMICS; IN-VIVO; PHOSPHORYLATION; TRANSCRIPTION; PLATFORM; STARS; TRANSDUCTION; MECHANISM; PATHWAY Multiple languages Physiology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/33257