Solagna, Francesca, Nogara, Leonardo, Dyar, Kenneth A., Greulich, Franziska, Mir, Ashfaq A., Turk, Clara, Bock, Theresa, Geremia, Alessia, Baraldo, Martina, Sartori, Roberta ORCID: 0000-0002-2763-9838, Farup, Jean ORCID: 0000-0002-7579-6512, Uhlenhaut, Henriette ORCID: 0000-0002-4220-4779, Vissing, Kristian, Krueger, Marcus and Blaauw, Bert (2020). Exercise-dependent increases in protein synthesis are accompanied by chromatin modifications and increased MRTF-SRF signalling. Acta Physiol., 230 (1). HOBOKEN: WILEY. ISSN 1748-1716

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Abstract

Aim Resistance exercise increases muscle mass over time. However, the early signalling events leading to muscle growth are not yet well-defined. Here, we aim to identify new signalling pathways important for muscle remodelling after exercise. Methods We performed a phosphoproteomics screen after a single bout of exercise in mice. As an exercise model we used unilateral electrical stimulation in vivo and treadmill running. We analysed muscle biopsies from human subjects to verify if our findings in murine muscle also translate to exercise in humans. Results We identified a new phosphorylation site on Myocardin-Related Transcription Factor B (MRTF-B), a co-activator of serum response factor (SRF). Phosphorylation of MRTF-B is required for its nuclear translocation after exercise and is accompanied by the transcription of the SRF target gene Fos. In addition, high-intensity exercise also remodels chromatin at specific SRF target gene loci through the phosphorylation of histone 3 on serine 10 in myonuclei of both mice and humans. Ablation of the MAP kinase member MSK1/2 is sufficient to prevent this histone phosphorylation, reduce induction of SRF-target genes, and prevent increases in protein synthesis after exercise. Conclusion Our results identify a new exercise signalling fingerprint in vivo, instrumental for exercise-induced protein synthesis and potentially muscle growth.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Solagna, FrancescaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Nogara, LeonardoUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Dyar, Kenneth A.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Greulich, FranziskaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Mir, Ashfaq A.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Turk, ClaraUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Bock, TheresaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Geremia, AlessiaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Baraldo, MartinaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Sartori, RobertaUNSPECIFIEDorcid.org/0000-0002-2763-9838UNSPECIFIED
Farup, JeanUNSPECIFIEDorcid.org/0000-0002-7579-6512UNSPECIFIED
Uhlenhaut, HenrietteUNSPECIFIEDorcid.org/0000-0002-4220-4779UNSPECIFIED
Vissing, KristianUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Krueger, MarcusUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Blaauw, BertUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-332578
DOI: 10.1111/apha.13496
Journal or Publication Title: Acta Physiol.
Volume: 230
Number: 1
Date: 2020
Publisher: WILEY
Place of Publication: HOBOKEN
ISSN: 1748-1716
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
HUMAN SKELETAL-MUSCLE; ACTIN DYNAMICS; IN-VIVO; PHOSPHORYLATION; TRANSCRIPTION; PLATFORM; STARS; TRANSDUCTION; MECHANISM; PATHWAYMultiple languages
PhysiologyMultiple languages
URI: http://kups.ub.uni-koeln.de/id/eprint/33257

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