Universität zu Köln

Jeanclos, Elisabeth, Knobloch, Gunnar, Hoffmann, Axel, Fedorchenko, Oleg, Odersky, Andrea, Lamprecht, Anna-Karina, Schindelin, Hermann and Gohla, Antje ORCID: 0000-0002-7442-1487 (2020). Ca2+ functions as a molecular switch that controls the mutually exclusive complex formation of pyridoxal phosphatase with CIB1 or calmodulin. FEBS Lett., 594 (13). S. 2099 - 2116. HOBOKEN: WILEY. ISSN 1873-3468

Full text not available from this repository.

Abstract

Pyridoxal 5 '-phosphate (PLP) is an essential cofactor for neurotransmitter metabolism. Pyridoxal phosphatase (PDXP) deficiency in mice increases PLP and gamma-aminobutyric acid levels in the brain, yet how PDXP is regulated is unclear. Here, we identify the Ca2+- and integrin-binding protein 1 (CIB1) as a PDXP interactor by yeast two-hybrid screening and find a calmodulin (CaM)-binding motif that overlaps with the PDXP-CIB1 interaction site. Pulldown and crosslinking assays with purified proteins demonstrate that PDXP directly binds to CIB1 or CaM. CIB1 or CaM does not alter PDXP phosphatase activity. However, elevated Ca2+ concentrations promote CaM binding and, thereby, diminish CIB1 binding to PDXP, as both interactors bind in a mutually exclusive way. Hence, the PDXP-CIB1 complex may functionally differ from the PDXP-Ca2+-CaM complex.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Jeanclos, Elisabeth UNSPECIFIED UNSPECIFIED UNSPECIFIED Knobloch, Gunnar UNSPECIFIED UNSPECIFIED UNSPECIFIED Hoffmann, Axel UNSPECIFIED UNSPECIFIED UNSPECIFIED Fedorchenko, Oleg UNSPECIFIED UNSPECIFIED UNSPECIFIED Odersky, Andrea UNSPECIFIED UNSPECIFIED UNSPECIFIED Lamprecht, Anna-Karina UNSPECIFIED UNSPECIFIED UNSPECIFIED Schindelin, Hermann UNSPECIFIED UNSPECIFIED UNSPECIFIED Gohla, Antje UNSPECIFIED orcid.org/0000-0002-7442-1487 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-333477
DOI:	10.1002/1873-3468.13795
Journal or Publication Title:	FEBS Lett.
Volume:	594
Number:	13
Page Range:	S. 2099 - 2116
Date:	2020
Publisher:	WILEY
Place of Publication:	HOBOKEN
ISSN:	1873-3468
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language INTEGRIN-BINDING PROTEIN-1; CALCIUM-BINDING; CHRONOPHIN; COFILIN; ACTIVATION; MECHANISM; COGNITION; MEMBRANE; KINASES; ENZYMES Multiple languages Biochemistry & Molecular Biology; Biophysics; Cell Biology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/33347