Kamrad, Stephan ORCID: 0000-0002-5957-4661, Grossbach, Jan, Rodriguez-Lopez, Maria, Muelleder, Michael, Townsend, StJohn, Cappelletti, Valentina, Stojanovski, Gorjan, Correia-Melo, Clara, Picotti, Paola, Beyer, Andreas ORCID: 0000-0002-3891-2123, Ralser, Markus ORCID: 0000-0001-9535-7413 and Bahler, Jurg ORCID: 0000-0003-4036-1532 (2020). Pyruvate kinase variant of fission yeast tunes carbon metabolism, cell regulation, growth and stress resistance. Mol. Syst. Biol., 16 (4). HOBOKEN: WILEY. ISSN 1744-4292

Full text not available from this repository.

Abstract

Cells balance glycolysis with respiration to support their metabolic needs in different environmental or physiological contexts. With abundant glucose, many cells prefer to grow by aerobic glycolysis or fermentation. Using 161 natural isolates of fission yeast, we investigated the genetic basis and phenotypic effects of the fermentation-respiration balance. The laboratory and a few other strains depended more on respiration. This trait was associated with a single nucleotide polymorphism in a conserved region of Pyk1, the sole pyruvate kinase in fission yeast. This variant reduced Pyk1 activity and glycolytic flux. Replacing the low-activity pyk1 allele in the laboratory strain with the high-activity allele was sufficient to increase fermentation and decrease respiration. This metabolic rebalancing triggered systems-level adjustments in the transcriptome and proteome and in cellular traits, including increased growth and chronological lifespan but decreased resistance to oxidative stress. Thus, low Pyk1 activity does not lead to a growth advantage but to stress tolerance. The genetic tuning of glycolytic flux may reflect an adaptive trade-off in a species lacking pyruvate kinase isoforms.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Kamrad, StephanUNSPECIFIEDorcid.org/0000-0002-5957-4661UNSPECIFIED
Grossbach, JanUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Rodriguez-Lopez, MariaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Muelleder, MichaelUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Townsend, StJohnUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Cappelletti, ValentinaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Stojanovski, GorjanUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Correia-Melo, ClaraUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Picotti, PaolaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Beyer, AndreasUNSPECIFIEDorcid.org/0000-0002-3891-2123UNSPECIFIED
Ralser, MarkusUNSPECIFIEDorcid.org/0000-0001-9535-7413UNSPECIFIED
Bahler, JurgUNSPECIFIEDorcid.org/0000-0003-4036-1532UNSPECIFIED
URN: urn:nbn:de:hbz:38-338551
Journal or Publication Title: Mol. Syst. Biol.
Volume: 16
Number: 4
Date: 2020
Publisher: WILEY
Place of Publication: HOBOKEN
ISSN: 1744-4292
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
CHRONOLOGICAL LIFE-SPAN; SCHIZOSACCHAROMYCES-POMBE; GENE-EXPRESSION; GLUCOSE-METABOLISM; ESCHERICHIA-COLI; OXIDATIVE STRESS; SACCHAROMYCES-CEREVISIAE; CALORIE RESTRICTION; OVERFLOW METABOLISM; AEROBIC GLYCOLYSISMultiple languages
Biochemistry & Molecular BiologyMultiple languages
URI: http://kups.ub.uni-koeln.de/id/eprint/33855

Downloads

Downloads per month over past year

Export

Actions (login required)

View Item View Item