Universität zu Köln

DiFrancesco, Mattia Lorenzo ORCID: 0000-0002-7511-4438, Lodola, Francesco ORCID: 0000-0002-3506-5619, Colombo, Elisabetta, Maragliano, Luca, Bramini, Mattia ORCID: 0000-0002-0381-9391, Paterno, Giuseppe Maria ORCID: 0000-0003-2349-566X, Baldelli, Pietro, Serra, Mauro Dalla ORCID: 0000-0003-1048-2739, Lunelli, Lorenzo, Marchioretto, Marta, Grasselli, Giorgio ORCID: 0000-0002-2942-9615, Cimo, Simone ORCID: 0000-0003-0333-5386, Colella, Letizia, Fazzi, Daniele ORCID: 0000-0002-8515-4214, Ortica, Fausto ORCID: 0000-0001-8276-452X, Vurro, Vito, Eleftheriou, Cyril Giles, Shmal, Dmytro ORCID: 0000-0001-6770-6364, Maya-Vetencourt, Jose Fernando ORCID: 0000-0003-3808-8944, Bertarelli, Chiara, Lanzani, Guglielmo and Benfenati, Fabio (2020). Neuronal firing modulation by a membrane-targeted photoswitch. Nat. Nanotechnol., 15 (4). S. 296 - 310. LONDON: NATURE PUBLISHING GROUP. ISSN 1748-3395

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Abstract

Light-sensitive azobenzene compounds can be engineered to stably partition into the plasma membrane, thus causing its thinning in the dark and relaxation upon light stimulation. In neurons, the resulting light-dependent change in membrane capacitance induces a transient hyperpolarization followed by rebound depolarization and action potential firing. Optical technologies allowing modulation of neuronal activity at high spatio-temporal resolution are becoming paramount in neuroscience. In this respect, azobenzene-based photoswitches are promising nanoscale tools for neuronal photostimulation. Here we engineered a light-sensitive azobenzene compound (Ziapin2) that stably partitions into the plasma membrane and causes its thinning through trans-dimerization in the dark, resulting in an increased membrane capacitance at steady state. We demonstrated that in neurons loaded with the compound, millisecond pulses of visible light induce a transient hyperpolarization followed by a delayed depolarization that triggers action potential firing. These effects are persistent and can be evoked in vivo up to 7 days, proving the potential of Ziapin2 for the modulation of membrane capacitance in the millisecond timescale, without directly affecting ion channels or local temperature.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code DiFrancesco, Mattia Lorenzo UNSPECIFIED orcid.org/0000-0002-7511-4438 UNSPECIFIED Lodola, Francesco UNSPECIFIED orcid.org/0000-0002-3506-5619 UNSPECIFIED Colombo, Elisabetta UNSPECIFIED UNSPECIFIED UNSPECIFIED Maragliano, Luca UNSPECIFIED UNSPECIFIED UNSPECIFIED Bramini, Mattia UNSPECIFIED orcid.org/0000-0002-0381-9391 UNSPECIFIED Paterno, Giuseppe Maria UNSPECIFIED orcid.org/0000-0003-2349-566X UNSPECIFIED Baldelli, Pietro UNSPECIFIED UNSPECIFIED UNSPECIFIED Serra, Mauro Dalla UNSPECIFIED orcid.org/0000-0003-1048-2739 UNSPECIFIED Lunelli, Lorenzo UNSPECIFIED UNSPECIFIED UNSPECIFIED Marchioretto, Marta UNSPECIFIED UNSPECIFIED UNSPECIFIED Grasselli, Giorgio UNSPECIFIED orcid.org/0000-0002-2942-9615 UNSPECIFIED Cimo, Simone UNSPECIFIED orcid.org/0000-0003-0333-5386 UNSPECIFIED Colella, Letizia UNSPECIFIED UNSPECIFIED UNSPECIFIED Fazzi, Daniele UNSPECIFIED orcid.org/0000-0002-8515-4214 UNSPECIFIED Ortica, Fausto UNSPECIFIED orcid.org/0000-0001-8276-452X UNSPECIFIED Vurro, Vito UNSPECIFIED UNSPECIFIED UNSPECIFIED Eleftheriou, Cyril Giles UNSPECIFIED UNSPECIFIED UNSPECIFIED Shmal, Dmytro UNSPECIFIED orcid.org/0000-0001-6770-6364 UNSPECIFIED Maya-Vetencourt, Jose Fernando UNSPECIFIED orcid.org/0000-0003-3808-8944 UNSPECIFIED Bertarelli, Chiara UNSPECIFIED UNSPECIFIED UNSPECIFIED Lanzani, Guglielmo UNSPECIFIED UNSPECIFIED UNSPECIFIED Benfenati, Fabio UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-338922
DOI:	10.1038/s41565-019-0632-6
Journal or Publication Title:	Nat. Nanotechnol.
Volume:	15
Number:	4
Page Range:	S. 296 - 310
Date:	2020
Publisher:	NATURE PUBLISHING GROUP
Place of Publication:	LONDON
ISSN:	1748-3395
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Chemistry > Institute of Physical Chemistry
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language PHOTOELECTRIC RESPONSE; LIGHT SENSITIVITY; ION CHANNELS; PHOTOISOMERIZATION; CAPACITANCE; INTERFACE; CELLS; MODEL; RAFT Multiple languages Nanoscience & Nanotechnology; Materials Science, Multidisciplinary Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/33892