DiFrancesco, Mattia Lorenzo ORCID: 0000-0002-7511-4438, Lodola, Francesco ORCID: 0000-0002-3506-5619, Colombo, Elisabetta, Maragliano, Luca, Bramini, Mattia ORCID: 0000-0002-0381-9391, Paterno, Giuseppe Maria ORCID: 0000-0003-2349-566X, Baldelli, Pietro, Serra, Mauro Dalla ORCID: 0000-0003-1048-2739, Lunelli, Lorenzo, Marchioretto, Marta, Grasselli, Giorgio ORCID: 0000-0002-2942-9615, Cimo, Simone ORCID: 0000-0003-0333-5386, Colella, Letizia, Fazzi, Daniele ORCID: 0000-0002-8515-4214, Ortica, Fausto ORCID: 0000-0001-8276-452X, Vurro, Vito, Eleftheriou, Cyril Giles, Shmal, Dmytro ORCID: 0000-0001-6770-6364, Maya-Vetencourt, Jose Fernando ORCID: 0000-0003-3808-8944, Bertarelli, Chiara, Lanzani, Guglielmo and Benfenati, Fabio (2020). Neuronal firing modulation by a membrane-targeted photoswitch. Nat. Nanotechnol., 15 (4). S. 296 - 310. LONDON: NATURE PUBLISHING GROUP. ISSN 1748-3395

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Abstract

Light-sensitive azobenzene compounds can be engineered to stably partition into the plasma membrane, thus causing its thinning in the dark and relaxation upon light stimulation. In neurons, the resulting light-dependent change in membrane capacitance induces a transient hyperpolarization followed by rebound depolarization and action potential firing. Optical technologies allowing modulation of neuronal activity at high spatio-temporal resolution are becoming paramount in neuroscience. In this respect, azobenzene-based photoswitches are promising nanoscale tools for neuronal photostimulation. Here we engineered a light-sensitive azobenzene compound (Ziapin2) that stably partitions into the plasma membrane and causes its thinning through trans-dimerization in the dark, resulting in an increased membrane capacitance at steady state. We demonstrated that in neurons loaded with the compound, millisecond pulses of visible light induce a transient hyperpolarization followed by a delayed depolarization that triggers action potential firing. These effects are persistent and can be evoked in vivo up to 7 days, proving the potential of Ziapin2 for the modulation of membrane capacitance in the millisecond timescale, without directly affecting ion channels or local temperature.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
DiFrancesco, Mattia LorenzoUNSPECIFIEDorcid.org/0000-0002-7511-4438UNSPECIFIED
Lodola, FrancescoUNSPECIFIEDorcid.org/0000-0002-3506-5619UNSPECIFIED
Colombo, ElisabettaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Maragliano, LucaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Bramini, MattiaUNSPECIFIEDorcid.org/0000-0002-0381-9391UNSPECIFIED
Paterno, Giuseppe MariaUNSPECIFIEDorcid.org/0000-0003-2349-566XUNSPECIFIED
Baldelli, PietroUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Serra, Mauro DallaUNSPECIFIEDorcid.org/0000-0003-1048-2739UNSPECIFIED
Lunelli, LorenzoUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Marchioretto, MartaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Grasselli, GiorgioUNSPECIFIEDorcid.org/0000-0002-2942-9615UNSPECIFIED
Cimo, SimoneUNSPECIFIEDorcid.org/0000-0003-0333-5386UNSPECIFIED
Colella, LetiziaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Fazzi, DanieleUNSPECIFIEDorcid.org/0000-0002-8515-4214UNSPECIFIED
Ortica, FaustoUNSPECIFIEDorcid.org/0000-0001-8276-452XUNSPECIFIED
Vurro, VitoUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Eleftheriou, Cyril GilesUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Shmal, DmytroUNSPECIFIEDorcid.org/0000-0001-6770-6364UNSPECIFIED
Maya-Vetencourt, Jose FernandoUNSPECIFIEDorcid.org/0000-0003-3808-8944UNSPECIFIED
Bertarelli, ChiaraUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Lanzani, GuglielmoUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Benfenati, FabioUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-338922
DOI: 10.1038/s41565-019-0632-6
Journal or Publication Title: Nat. Nanotechnol.
Volume: 15
Number: 4
Page Range: S. 296 - 310
Date: 2020
Publisher: NATURE PUBLISHING GROUP
Place of Publication: LONDON
ISSN: 1748-3395
Language: English
Faculty: Faculty of Mathematics and Natural Sciences
Divisions: Faculty of Mathematics and Natural Sciences > Department of Chemistry > Institute of Physical Chemistry
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
PHOTOELECTRIC RESPONSE; LIGHT SENSITIVITY; ION CHANNELS; PHOTOISOMERIZATION; CAPACITANCE; INTERFACE; CELLS; MODEL; RAFTMultiple languages
Nanoscience & Nanotechnology; Materials Science, MultidisciplinaryMultiple languages
Refereed: Yes
URI: http://kups.ub.uni-koeln.de/id/eprint/33892

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