Universität zu Köln

Reinking, Hannah K., Hofmann, Kay ORCID: 0000-0002-2289-9083 and Stingele, Julian ORCID: 0000-0001-6020-2075 (2020). Function and evolution of the DNA-protein crosslink proteases Wss1 and SPRTN. DNA Repair, 88. AMSTERDAM: ELSEVIER. ISSN 1568-7856

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Abstract

Covalent DNA-protein crosslinks (DPCs) are highly toxic DNA adducts, which interfere with faithful DNA replication. The proteases Wss1 and SPRTN degrade DPCs and have emerged as crucially important DNA repair enzymes. Their protective role has been described in various model systems ranging from yeasts, plants, worms and flies to mice and humans. Loss of DPC proteases results in genome instability, cellular arrest, premature ageing and cancer predisposition. Here we discuss recent insights into the function and molecular mechanism of these enzymes. Furthermore, we present an in-depth phylogenetic analysis of the Wss1/SPRTN protease continuum. Remarkably flexible domain architectures and constantly changing protein-protein interaction motifs indicate ongoing evolutionary dynamics. Finally, we discuss recent data, which suggest that further partially-overlapping proteolytic systems targeting DPCs exist in eukaryotes. These new developments raise interesting questions regarding the division of labour between different DPC proteases and the mechanisms and principles of repair pathway choice.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Reinking, Hannah K. UNSPECIFIED UNSPECIFIED UNSPECIFIED Hofmann, Kay UNSPECIFIED orcid.org/0000-0002-2289-9083 UNSPECIFIED Stingele, Julian UNSPECIFIED orcid.org/0000-0001-6020-2075 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-339751
DOI:	10.1016/j.dnarep.2020.102822
Journal or Publication Title:	DNA Repair
Volume:	88
Date:	2020
Publisher:	ELSEVIER
Place of Publication:	AMSTERDAM
ISSN:	1568-7856
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Biology > Institute for Genetics
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language NUCLEOTIDE EXCISION-REPAIR; HISTONE DEMETHYLATION; TOPOISOMERASE-II; DVC1 C1ORF124; REPLICATION; SPARTAN; SPARTAN/C1ORF124; MECHANISM; GENE; P97 Multiple languages Genetics & Heredity; Toxicology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/33975