Universität zu Köln

Gebauer, Jan M., Flachsenberg, Florian ORCID: 0000-0001-7051-8719, Windler, Cordula, Richer, Barbara, Baumann, Ulrich and Seeger, Karsten (2020). Structural and biophysical characterization of the type VII collagen vWFA2 subdomain leads to identification of two binding sites. FEBS Open Bio, 10 (4). S. 580 - 593. HOBOKEN: WILEY. ISSN 2211-5463

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Abstract

Type VII collagen is an extracellular matrix protein, which is important for skin stability; however, detailed information at the molecular level is scarce. The second vWFA (von Willebrand factor type A) domain of type VII collagen mediates important interactions, and immunization of mice induces skin blistering in certain strains. To understand vWFA2 function and the pathophysiological mechanisms leading to skin blistering, we structurally characterized this domain by X-ray crystallography and NMR spectroscopy. Cell adhesion assays identified two new interactions: one with beta 1 integrin via its RGD motif and one with laminin-332. The latter interaction was confirmed by surface plasmon resonance with a K-D of about 1 mm. These data show that vWFA2 has additional functions in the extracellular matrix besides interacting with type I collagen.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Gebauer, Jan M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Flachsenberg, Florian UNSPECIFIED orcid.org/0000-0001-7051-8719 UNSPECIFIED Windler, Cordula UNSPECIFIED UNSPECIFIED UNSPECIFIED Richer, Barbara UNSPECIFIED UNSPECIFIED UNSPECIFIED Baumann, Ulrich UNSPECIFIED UNSPECIFIED UNSPECIFIED Seeger, Karsten UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-340979
DOI:	10.1002/2211-5463.12807
Journal or Publication Title:	FEBS Open Bio
Volume:	10
Number:	4
Page Range:	S. 580 - 593
Date:	2020
Publisher:	WILEY
Place of Publication:	HOBOKEN
ISSN:	2211-5463
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Chemistry > Institute of Biochemistry
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language EPIDERMOLYSIS-BULLOSA ACQUISITA; PROTEIN SECONDARY STRUCTURE; NC1 DOMAIN; NMR; ALIGNMENT; MODEL; PREDICTION; LAMININ-5; FIBRONECTIN; REGION Multiple languages Biochemistry & Molecular Biology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/34097