Universität zu Köln

Mannaa, Atef and Hanisch, Franz-Georg (2020). Redox Proteomes in Human Physiology and Disease Mechanisms. J. Proteome Res., 19 (1). S. 1 - 18. WASHINGTON: AMER CHEMICAL SOC. ISSN 1535-3907

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Abstract

Redox proteomics is a field of proteomics that is concerned with the characterization of the oxidation state of proteins to gain information about their modulated structure, function, activity, and involvement in different physiological pathways. Oxidative modifications of proteins have been shown to be implicated in normal physiological processes of cells as well as in pathomechanisms leading to the development of cancer, diabetes, neurodegenerative diseases, and some rare hereditary metabolic diseases, like classic galactosemia. Reactive oxygen species generate a variety of reversible and irreversible modifications in amino acid residue side chains and within the protein backbone. These oxidative post-translational modifications (Ox-PTMs) can participate in the activation of signal transduction pathways and mediate the toxicity of harmful oxidants. Thus the application of advanced redox proteomics technologies is important for gaining insights into molecular mechanisms of diseases. Mass-spectrometry-based proteomics is one of the most powerful methods that can be used to give detailed qualitative and quantitative information on protein modifications and allows us to characterize redox proteomes associated with diseases. This Review illustrates the role and biological consequences of Ox-PTMs under basal and oxidative stress conditions by focusing on protein carbonylation and S-glutathionylation, two abundant modifications with an impact on cellular pathways that have been intensively studied during the past decade.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Mannaa, Atef UNSPECIFIED UNSPECIFIED UNSPECIFIED Hanisch, Franz-Georg UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-352187
DOI:	10.1021/acs.jproteome.9b00586
Journal or Publication Title:	J. Proteome Res.
Volume:	19
Number:	1
Page Range:	S. 1 - 18
Date:	2020
Publisher:	AMER CHEMICAL SOC
Place of Publication:	WASHINGTON
ISSN:	1535-3907
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language PROTEIN S-GLUTATHIONYLATION; OXIDATIVELY MODIFIED PROTEINS; CYSTEINE SULFENIC ACID; GLYCATION END-PRODUCTS; FACTOR-KAPPA-B; MASS-SPECTROMETRY; CARBONYLATED PROTEINS; REACTIVE OXYGEN; QUANTITATIVE PROTEOMICS; CELLULAR DYSFUNCTION Multiple languages Biochemical Research Methods Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/35218