Universität zu Köln

Huebbers, Christian U., Adam, Alexander C., Preuss, Simon F., Schiffer, Theresa, Schilder, Sarah, Guntinas-Lichius, Orlando ORCID: 0000-0001-9671-0784, Schmidt, Matthias, Klussmann, Jens P. and Wiesner, Rudolf J. (2015). High glucose uptake unexpectedly is accompanied by high levels of the mitochondrial beta-F1-ATPase subunit in head and neck squamous cell carcinoma. Oncotarget, 6 (34). S. 36172 - 36185. ORCHARD PARK: IMPACT JOURNALS LLC. ISSN 1949-2553

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Abstract

A hallmark of solid tumors is the consumption of large amounts of glucose and production of lactate, also known as Warburg-like metabolism. This metabolic phenotype is typical for aggressive tumor growth, and can be visualized by F-18-fluorodeoxyglucose (F-18-FDG) uptake detected by positron emission tomography (PET). High F-18-FDG uptake inversely correlates with survival and goes along with reduced expression of the catalytic beta-subunit of the H+-ATP synthase (beta-F1-ATPase) in several tumor entities analyzed so far. For this study we characterized a series of 15 head and neck squamous cell carcinoma (HNSCC) by (i) determining F-18-FDG-uptake; (ii) quantitative expression analysis of beta-F1-ATPase (Complex V), NDUF-S1 (Complex I) and COX1 (Complex IV) of the mitochondrial electron transport chain (ETC), as well as Hsp60 (mitochondrial mass) and GAPDH (glycolysis) in tumor cells; (iii) sequencing of the mtDNA of representative tumor samples. Whereas high F-18-FDG-uptake also correlates with poor prognosis in HNSCC, it surprisingly is accompanied by high levels of beta-F1-ATPase, but not by any of the other analyzed proteins. In conclusion, we here describe a completely new phenotype of metabolic adaptation possibly enabling those tumors with highest levels of beta-F1-ATPase to rapidly proliferate even in hypoxic zones, which are typical for HNSCC.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Huebbers, Christian U. UNSPECIFIED UNSPECIFIED UNSPECIFIED Adam, Alexander C. UNSPECIFIED UNSPECIFIED UNSPECIFIED Preuss, Simon F. UNSPECIFIED UNSPECIFIED UNSPECIFIED Schiffer, Theresa UNSPECIFIED UNSPECIFIED UNSPECIFIED Schilder, Sarah UNSPECIFIED UNSPECIFIED UNSPECIFIED Guntinas-Lichius, Orlando UNSPECIFIED orcid.org/0000-0001-9671-0784 UNSPECIFIED Schmidt, Matthias UNSPECIFIED UNSPECIFIED UNSPECIFIED Klussmann, Jens P. UNSPECIFIED UNSPECIFIED UNSPECIFIED Wiesner, Rudolf J. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-387256
DOI:	10.18632/oncotarget.5459
Journal or Publication Title:	Oncotarget
Volume:	6
Number:	34
Page Range:	S. 36172 - 36185
Date:	2015
Publisher:	IMPACT JOURNALS LLC
Place of Publication:	ORCHARD PARK
ISSN:	1949-2553
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language ATPASE INHIBITORY FACTOR-1; CANCER-CELLS; METABOLISM; GLYCOLYSIS; MUTATIONS; MARKERS; CHAIN; DNA; BIOGENESIS; PHYSIOLOGY Multiple languages Oncology; Cell Biology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/38725