Huebbers, Christian U., Adam, Alexander C., Preuss, Simon F., Schiffer, Theresa, Schilder, Sarah, Guntinas-Lichius, Orlando ORCID: 0000-0001-9671-0784, Schmidt, Matthias, Klussmann, Jens P. and Wiesner, Rudolf J. (2015). High glucose uptake unexpectedly is accompanied by high levels of the mitochondrial beta-F1-ATPase subunit in head and neck squamous cell carcinoma. Oncotarget, 6 (34). S. 36172 - 36185. ORCHARD PARK: IMPACT JOURNALS LLC. ISSN 1949-2553

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Abstract

A hallmark of solid tumors is the consumption of large amounts of glucose and production of lactate, also known as Warburg-like metabolism. This metabolic phenotype is typical for aggressive tumor growth, and can be visualized by F-18-fluorodeoxyglucose (F-18-FDG) uptake detected by positron emission tomography (PET). High F-18-FDG uptake inversely correlates with survival and goes along with reduced expression of the catalytic beta-subunit of the H+-ATP synthase (beta-F1-ATPase) in several tumor entities analyzed so far. For this study we characterized a series of 15 head and neck squamous cell carcinoma (HNSCC) by (i) determining F-18-FDG-uptake; (ii) quantitative expression analysis of beta-F1-ATPase (Complex V), NDUF-S1 (Complex I) and COX1 (Complex IV) of the mitochondrial electron transport chain (ETC), as well as Hsp60 (mitochondrial mass) and GAPDH (glycolysis) in tumor cells; (iii) sequencing of the mtDNA of representative tumor samples. Whereas high F-18-FDG-uptake also correlates with poor prognosis in HNSCC, it surprisingly is accompanied by high levels of beta-F1-ATPase, but not by any of the other analyzed proteins. In conclusion, we here describe a completely new phenotype of metabolic adaptation possibly enabling those tumors with highest levels of beta-F1-ATPase to rapidly proliferate even in hypoxic zones, which are typical for HNSCC.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Huebbers, Christian U.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Adam, Alexander C.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Preuss, Simon F.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Schiffer, TheresaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Schilder, SarahUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Guntinas-Lichius, OrlandoUNSPECIFIEDorcid.org/0000-0001-9671-0784UNSPECIFIED
Schmidt, MatthiasUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Klussmann, Jens P.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Wiesner, Rudolf J.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-387256
DOI: 10.18632/oncotarget.5459
Journal or Publication Title: Oncotarget
Volume: 6
Number: 34
Page Range: S. 36172 - 36185
Date: 2015
Publisher: IMPACT JOURNALS LLC
Place of Publication: ORCHARD PARK
ISSN: 1949-2553
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
ATPASE INHIBITORY FACTOR-1; CANCER-CELLS; METABOLISM; GLYCOLYSIS; MUTATIONS; MARKERS; CHAIN; DNA; BIOGENESIS; PHYSIOLOGYMultiple languages
Oncology; Cell BiologyMultiple languages
URI: http://kups.ub.uni-koeln.de/id/eprint/38725

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