Universität zu Köln

Badia, Mariana B., Arias, Cintia L., Tronconi, Marcos A., Maurino, Veronica G., Andreo, Carlos S., Drincovich, Maria F. and Gerrard Wheeler, Mariel C. (2015). Enhanced cytosolic NADP-ME2 activity in A-thaliana affects plant development, stress tolerance and specific diurnal and nocturnal cellular processes. Plant Sci., 240. S. 193 - 204. CLARE: ELSEVIER IRELAND LTD. ISSN 0168-9452

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Abstract

Arabidopsis thaliana has four NADP-dependent malic enzymes (NADP-ME 1-4) for reversible malate decarboxylation, with NADP-ME2 being the only cytosolic isoform ubiquitously expressed and responsible for most of the total activity. In this work, we further investigated its physiological function by characterizing Arabidopsis plants over-expressing NADP-ME2 constitutively. In comparison to wild type, these plants exhibited reduced rosette and root sizes, delayed flowering time and increased sensitivity to mannitol and polyethylene glycol. The increased NADP-ME2 activity led to a decreased expression of other ME and malate dehydrogenase isoforms and generated a redox imbalance with opposite characteristics depending on the time point of the day analyzed. The over-expressing plants also presented a higher content of C-4 organic acids and sugars under normal growth conditions. However, the accumulation of these metabolites in the over-expressing plants was substantially less pronounced after osmotic stress exposure compared to wild type. Also, a lower level of several amino acids and osmoprotector compounds was observed in transgenic plants. Thus, the gain of NADP-ME2 expression has profound consequences in the modulation of primary metabolism in A. thaliana, which reflect the relevance of this enzyme and its substrates and products in plant homeostasis. (C) 2015 Elsevier Ireland Ltd. All rights reserved.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Badia, Mariana B. UNSPECIFIED UNSPECIFIED UNSPECIFIED Arias, Cintia L. UNSPECIFIED UNSPECIFIED UNSPECIFIED Tronconi, Marcos A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Maurino, Veronica G. UNSPECIFIED UNSPECIFIED UNSPECIFIED Andreo, Carlos S. UNSPECIFIED UNSPECIFIED UNSPECIFIED Drincovich, Maria F. UNSPECIFIED UNSPECIFIED UNSPECIFIED Gerrard Wheeler, Mariel C. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-388274
DOI:	10.1016/j.plantsci.2015.09.015
Journal or Publication Title:	Plant Sci.
Volume:	240
Page Range:	S. 193 - 204
Date:	2015
Publisher:	ELSEVIER IRELAND LTD
Place of Publication:	CLARE
ISSN:	0168-9452
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language PEROXISOMAL MALATE-DEHYDROGENASE; NAD-MALIC ENZYMES; RELATIVE QUANTIFICATION; OXIDATIVE STRESS; ORGANIC-ACIDS; ARABIDOPSIS; METABOLISM; FUMARATE; IDENTIFICATION; TRANSPORTER Multiple languages Biochemistry & Molecular Biology; Plant Sciences Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/38827