Universität zu Köln

Alnawaiseh, M., Albanna, W., Abumuaileq, R., Boehm, M. R. R., Eter, N. and Schneider, T. (2015). Effect of Hypericin on the Function of the Neuroretina: An Electroretinographic Study. Klinische Monatsblat. Augenheilkunde, 232 (11). S. 1304 - 1308. STUTTGART: GEORG THIEME VERLAG KG. ISSN 1439-3999

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Abstract

Background: Hypericin is an important component of the Saint John's wort (Hypericum perforatum). It is assumed to inhibit intracellular signalling cascades, which contribute to neoangiogenesis. The phototoxic effect of hypericin on the retina was investigated in human retinal pigment epithelium (RPE); hypericin induces oxidative stress and has also been described to be an inhibitor of Ca2+ influx channel in cultured RPE cells. The aim of our study is to evaluate the effect of hypericine on the function of the neuroretina. Methods: Isolated bovine retinas were perfused with an oxygen saturated nutrient solution (1 mL/min). We exposed the retina to a flashlight of 6.3 mlx every 5 min. The electroretinogram (ERG) was recorded as a transretinal potential using Ag/AgCl electrodes. ERGs were monitored before, during and after hypericin exposure. Results: In three independent experiments we investigated the effect of hypericin on the amplitude of the b-wave. In our experiments we observed a significant reduction of the amplitude of the b-wave to 87.1 +/- 3.5% (p = 0.02). This reduction was in all our experiments partially reversible. After hypericin wash-out the b-wave amplitude did not recover completely and did not return to the initial value (91.0 +/- 5.1%; not significant). We did not observe a significant effect of hypericin on the implicit time of the b-wave. Conclusion: This study shows for the first time that hypericin influences retinal signal transduction, suggesting that hypericin impairs not only the RPE, but also affects retinal signalling and function.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Alnawaiseh, M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Albanna, W. UNSPECIFIED UNSPECIFIED UNSPECIFIED Abumuaileq, R. UNSPECIFIED UNSPECIFIED UNSPECIFIED Boehm, M. R. R. UNSPECIFIED UNSPECIFIED UNSPECIFIED Eter, N. UNSPECIFIED UNSPECIFIED UNSPECIFIED Schneider, T. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-388872
DOI:	10.1055/s-0035-1546157
Journal or Publication Title:	Klinische Monatsblat. Augenheilkunde
Volume:	232
Number:	11
Page Range:	S. 1304 - 1308
Date:	2015
Publisher:	GEORG THIEME VERLAG KG
Place of Publication:	STUTTGART
ISSN:	1439-3999
Language:	German
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language PERFORATUM; CANCER; CELLS Multiple languages Ophthalmology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/38887