Universität zu Köln

Geisen, Stefan ORCID: 0000-0003-0734-727X, Tveit, Alexander T., Clark, Ian M., Richter, Andreas ORCID: 0000-0003-3282-4808, Svenning, Mette M., Bonkowski, Michael ORCID: 0000-0003-2656-1183 and Urich, Tim ORCID: 0000-0002-5718-1354 (2015). Metatranscriptomic census of active protists in soils. ISME J., 9 (10). S. 2178 - 2191. LONDON: NATURE PUBLISHING GROUP. ISSN 1751-7370

Full text not available from this repository.

Abstract

The high numbers and diversity of protists in soil systems have long been presumed, but their true diversity and community composition have remained largely concealed. Traditional cultivation-based methods miss a majority of taxa, whereas molecular barcoding approaches employing PCR introduce significant biases in reported community composition of soil protists. Here, we applied a metatranscriptomic approach to assess the protist community in 12 mineral and organic soil samples from different vegetation types and climatic zones using small subunit ribosomal RNA transcripts as marker. We detected a broad diversity of soil protists spanning across all known eukaryotic supergroups and revealed a strikingly different community composition than shown before. Protist communities differed strongly between sites, with Rhizaria and Amoebozoa dominating in forest and grassland soils, while Alveolata were most abundant in peat soils. The Amoebozoa were comprised of Tubulinea, followed with decreasing abundance by Discosea, Variosea and Mycetozoa. Transcripts of Oomycetes, Apicomplexa and Ichthyosporea suggest soil as reservoir of parasitic protist taxa. Further, Foraminifera and Choanoflagellida were ubiquitously detected, showing that these typically marine and freshwater protists are autochthonous members of the soil microbiota. To the best of our knowledge, this metatranscriptomic study provides the most comprehensive picture of active protist communities in soils to date, which is essential to target the ecological roles of protists in the complex soil system.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Geisen, Stefan UNSPECIFIED orcid.org/0000-0003-0734-727X UNSPECIFIED Tveit, Alexander T. UNSPECIFIED UNSPECIFIED UNSPECIFIED Clark, Ian M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Richter, Andreas UNSPECIFIED orcid.org/0000-0003-3282-4808 UNSPECIFIED Svenning, Mette M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Bonkowski, Michael UNSPECIFIED orcid.org/0000-0003-2656-1183 UNSPECIFIED Urich, Tim UNSPECIFIED orcid.org/0000-0002-5718-1354 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-392050
DOI:	10.1038/ismej.2015.30
Journal or Publication Title:	ISME J.
Volume:	9
Number:	10
Page Range:	S. 2178 - 2191
Date:	2015
Publisher:	NATURE PUBLISHING GROUP
Place of Publication:	LONDON
ISSN:	1751-7370
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language MICROBIAL COMMUNITY STRUCTURE; BELOWGROUND FOOD WEBS; FREE-LIVING AMEBAS; BALAMUTHIA-MANDRILLARIS; REVISED CLASSIFICATION; MOLECULAR PHYLOGENY; NAEGLERIA-FOWLERI; GENETIC DIVERSITY; HIGHLY DIVERSE; FRESH-WATER Multiple languages Ecology; Microbiology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/39205