Universität zu Köln

Hoefler, Saskia, Glasenapp, Yvana, Thangaradjou, Thirunavukarassu, Lucas, Christina ORCID: 0000-0002-6575-3877 and Papenbrock, Jutta (2015). New insights into DNA barcoding of seagrasses. Syst. Biodivers., 13 (5). S. 496 - 509. ABINGDON: TAYLOR & FRANCIS LTD. ISSN 1478-0933

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Abstract

Taxonomists find some plant genera challenging because of the few morphological differences or unclear characters among closely related species, which leads to the misidentification of taxa. DNA barcoding is an approach to identify species by using short orthologous DNA sequences, known as DNA barcodes'. Concatenated rbcL and matK sequences are considered DNA barcodes for seagrasses. However, these markers are not applicable to all members of seagrasses at the species level, especially within the genus Halophila. Our previous studies indicated that the internal transcribed spacer (ITS) showed higher species resolution than the concatenated rbcL and matK sequences in the case of Halophila ovalis and closely related species. In this study, 26 ITS, two rbcL and two matK consensus sequences from 18 seagrass taxa belonging to four families collected in India, Vietnam, Germany, Croatia and Egypt were processed. Molecular ITS analysis resolved five clades. The results also indicate that the Cymodoceaceae family might be a non-monophyletic group. In conclusion, ITS could be applied as a DNA barcode for seagrasses instead of the rbcL/matK system previously proposed.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Hoefler, Saskia UNSPECIFIED UNSPECIFIED UNSPECIFIED Glasenapp, Yvana UNSPECIFIED UNSPECIFIED UNSPECIFIED Thangaradjou, Thirunavukarassu UNSPECIFIED UNSPECIFIED UNSPECIFIED Lucas, Christina UNSPECIFIED orcid.org/0000-0002-6575-3877 UNSPECIFIED Papenbrock, Jutta UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-393209
DOI:	10.1080/14772000.2015.1046408
Journal or Publication Title:	Syst. Biodivers.
Volume:	13
Number:	5
Page Range:	S. 496 - 509
Date:	2015
Publisher:	TAYLOR & FRANCIS LTD
Place of Publication:	ABINGDON
ISSN:	1478-0933
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language ROOTED PHYLOGENETIC TREES; GENUS HALOPHILA THOUARS; HYDROCHARITACEAE; ZOSTERACEAE; DIVERSIFICATION; CYMODOCEACEAE; EVOLUTION; NUCLEAR; OVALIS; RBCL Multiple languages Biodiversity Conservation; Biology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/39320