Universität zu Köln

Huedig, Meike, Maier, Alexander, Scherrers, Isabell, Seidel, Laura, Jansen, Erwin E. W., Mettler-Altmann, Tabea, Engqvist, Martin K. M. and Maurino, Veronica G. (2015). Plants Possess a Cyclic Mitochondrial Metabolic Pathway similar to the Mammalian Metabolic Repair Mechanism Involving Malate Dehydrogenase and l-2-Hydroxyglutarate Dehydrogenase. Plant Cell Physiol., 56 (9). S. 1820 - 1831. OXFORD: OXFORD UNIV PRESS. ISSN 1471-9053

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Abstract

Enzymatic side reactions can give rise to the formation of wasteful and toxic products that are removed by metabolite repair pathways. In this work, we identify and characterize a mitochondrial metabolic repair mechanism in Arabidopsis thaliana involving malate dehydrogenase (mMDH) and l-2-hydroxyglutarate dehydrogenase (l-2HGDH). We analyze the kinetic properties of both A. thaliana mMDH isoforms, and show that they produce l-2-hydroxyglutarate (l-2HG) from 2-ketoglutarate (2-KG) at low rates in side reactions. We identify A. thaliana l-2HGDH as a mitochondrial FAD-containing oxidase that converts l-2HG back to 2-KG. Using loss-of-function mutants, we show that the electrons produced in the l-2HGDH reaction are transferred to the mitochondrial electron transport chain through the electron transfer protein (ETF). Thus, plants possess the biochemical components of an l-2HG metabolic repair system identical to that found in mammals. While deficiencies in the metabolism of l-2HG result in fatal disorders in mammals, accumulation of l-2HG in plants does not adversely affect their development under a range of tested conditions. However, orthologs of l-2HGDH are found in all examined genomes of viridiplantae, indicating that the repair reaction we identified makes an essential contribution to plant fitness in as yet unidentified conditions in the wild.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Huedig, Meike UNSPECIFIED UNSPECIFIED UNSPECIFIED Maier, Alexander UNSPECIFIED UNSPECIFIED UNSPECIFIED Scherrers, Isabell UNSPECIFIED UNSPECIFIED UNSPECIFIED Seidel, Laura UNSPECIFIED UNSPECIFIED UNSPECIFIED Jansen, Erwin E. W. UNSPECIFIED UNSPECIFIED UNSPECIFIED Mettler-Altmann, Tabea UNSPECIFIED UNSPECIFIED UNSPECIFIED Engqvist, Martin K. M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Maurino, Veronica G. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-395841
DOI:	10.1093/pcp/pcv108
Journal or Publication Title:	Plant Cell Physiol.
Volume:	56
Number:	9
Page Range:	S. 1820 - 1831
Date:	2015
Publisher:	OXFORD UNIV PRESS
Place of Publication:	OXFORD
ISSN:	1471-9053
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language ELECTRON-TRANSFER FLAVOPROTEIN; ARABIDOPSIS-THALIANA; TRANSPORT CHAIN; IDENTIFICATION; ACIDURIA; 2-HYDROXYGLUTARATE; OXIDATION; PHOTORESPIRATION; FORMS Multiple languages Plant Sciences; Cell Biology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/39584