Universität zu Köln

Stange, Richard ORCID: 0000-0003-0807-3151, Sahin, Hacer, Wieskoetter, Britta, Persigehl, Thorsten, Ring, Janine, Bremer, Christoph, Raschke, Michael J. and Vieth, Volker (2015). In vivo monitoring of angiogenesis during tendon repair: a novel MRI-based technique in a rat patellar tendon model. Knee Surg. Sports Traumatol. Arthrosc., 23 (8). S. 2433 - 2440. NEW YORK: SPRINGER. ISSN 1433-7347

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Abstract

Recent in vivo studies were able to show the impairing effect of neoangiogenesis in degenerative tendon diseases. Clinical in vivo monitoring of angiogenesis in injured tendons therefore seems to be crucial for an accurate therapeutic approach. The aim of this study was to develop a novel magnetic resonance imaging (MRI)-based technique for observing angiogenesis during tendon healing in vivo. Tendinopathy was induced by an in situ freezing model of rat patellar tendon and monitored after 7, 14, and 28 days. Animals were randomly divided into an imaging and immunohistochemical group. MRI with a 'blood pool' contrast agent was used to determine neoangiogenesis during tendon healing. MRI was compared to histochemical staining and quantification of blood vessels in injured and native tendons. MRI data revealed a peak in changes in the transverse relaxation rate (Delta R (2)*), which is proportional to relative blood volume, 7 days after surgery and decrease until day 28. Histological microvessel density and vascular endothelial growth factor synthesis were also most evident at day 7 and decreased over time. The current results are demonstrating a time-dependent correlation between microvessel density and Delta R (2)*. Thus, MRI-based evaluation of angiogenesis in the tendon might be a new promising technique for in vivo monitoring of angiogenesis and therapy response in the future.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Stange, Richard UNSPECIFIED orcid.org/0000-0003-0807-3151 UNSPECIFIED Sahin, Hacer UNSPECIFIED UNSPECIFIED UNSPECIFIED Wieskoetter, Britta UNSPECIFIED UNSPECIFIED UNSPECIFIED Persigehl, Thorsten UNSPECIFIED UNSPECIFIED UNSPECIFIED Ring, Janine UNSPECIFIED UNSPECIFIED UNSPECIFIED Bremer, Christoph UNSPECIFIED UNSPECIFIED UNSPECIFIED Raschke, Michael J. UNSPECIFIED UNSPECIFIED UNSPECIFIED Vieth, Volker UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-397778
DOI:	10.1007/s00167-014-2897-5
Journal or Publication Title:	Knee Surg. Sports Traumatol. Arthrosc.
Volume:	23
Number:	8
Page Range:	S. 2433 - 2440
Date:	2015
Publisher:	SPRINGER
Place of Publication:	NEW YORK
ISSN:	1433-7347
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language ENDOTHELIAL GROWTH-FACTOR; BLOOD-VOLUME; TUMOR ANGIOGENESIS; COLOR DOPPLER; FACTOR VEGF; ACHILLES; EXPRESSION; CONTRAST; TENDINOPATHY; HYPOXIA Multiple languages Orthopedics; Sport Sciences; Surgery Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/39777