Universität zu Köln

Petersen, Hendrik O., Hoeger, Stefanie K., Looso, Mario ORCID: 0000-0003-1495-9530, Lengfeld, Tobias, Kuhn, Anne, Warnken, Uwe, Nishimiya-Fujisawa, Chiemi, Schnoelzer, Martina, Krueger, Marcus ORCID: 0000-0003-2008-4582, Oezbek, Suat, Simakov, Oleg ORCID: 0000-0002-3585-4511 and Holstein, Thomas W. (2015). A Comprehensive Transcriptomic and Proteomic Analysis of Hydra Head Regeneration. Mol. Biol. Evol., 32 (8). S. 1928 - 1948. OXFORD: OXFORD UNIV PRESS. ISSN 1537-1719

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Abstract

The cnidarian freshwater polyp Hydra sp. exhibits an unparalleled regeneration capacity in the animal kingdom. Using an integrative transcriptomic and stable isotope labeling by amino acids in cell culture proteomic/phosphoproteomic approach, we studied stem cell-based regeneration in Hydra polyps. As major contributors to head regeneration, we identified diverse signaling pathways adopted for the regeneration response as well as enriched novel genes. Our global analysis reveals two distinct molecular cascades: an early injury response and a subsequent, signaling driven patterning of the regenerating tissue. A key factor of the initial injury response is a general stabilization of proteins and a net upregulation of transcripts, which is followed by a subsequent activation cascade of signaling molecules including Wnts and transforming growth factor (TGF) beta-related factors. We observed moderate overlap between the factors contributing to proteomic and transcriptomic responses suggesting a decoupled regulation between the transcriptional and translational levels. Our data also indicate that interstitial stem cells and their derivatives (e.g., neurons) have no major role in Hydra head regeneration. Remarkably, we found an enrichment of evolutionarily more recent genes in the early regeneration response, whereas conserved genes are more enriched in the late phase. In addition, genes specific to the early injury response were enriched in transposon insertions. Genetic dynamicity and taxon-specific factors might therefore play a hitherto underestimated role in Hydra regeneration.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Petersen, Hendrik O. UNSPECIFIED UNSPECIFIED UNSPECIFIED Hoeger, Stefanie K. UNSPECIFIED UNSPECIFIED UNSPECIFIED Looso, Mario UNSPECIFIED orcid.org/0000-0003-1495-9530 UNSPECIFIED Lengfeld, Tobias UNSPECIFIED UNSPECIFIED UNSPECIFIED Kuhn, Anne UNSPECIFIED UNSPECIFIED UNSPECIFIED Warnken, Uwe UNSPECIFIED UNSPECIFIED UNSPECIFIED Nishimiya-Fujisawa, Chiemi UNSPECIFIED UNSPECIFIED UNSPECIFIED Schnoelzer, Martina UNSPECIFIED UNSPECIFIED UNSPECIFIED Krueger, Marcus UNSPECIFIED orcid.org/0000-0003-2008-4582 UNSPECIFIED Oezbek, Suat UNSPECIFIED UNSPECIFIED UNSPECIFIED Simakov, Oleg UNSPECIFIED orcid.org/0000-0002-3585-4511 UNSPECIFIED Holstein, Thomas W. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-398209
DOI:	10.1093/molbev/msv079
Journal or Publication Title:	Mol. Biol. Evol.
Volume:	32
Number:	8
Page Range:	S. 1928 - 1948
Date:	2015
Publisher:	OXFORD UNIV PRESS
Place of Publication:	OXFORD
ISSN:	1537-1719
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Biology > Institute for Genetics
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language CONTROLS DNA-REPLICATION; DEVELOPMENTAL MECHANISMS; NEGATIVE REGULATORS; LIMB REGENERATION; GENETIC-ANALYSIS; HAIR FOLLICLE; CELL LINEAGES; AMINO-ACIDS; PROTEIN; FAMILY Multiple languages Biochemistry & Molecular Biology; Evolutionary Biology; Genetics & Heredity Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/39820