Universität zu Köln

Li, Jing, Lehmann, Clara ORCID: 0000-0002-7042-1578, Chen, Xishan, Romerio, Fabio and Lu, Wuyuan ORCID: 0000-0003-1318-9968 (2015). Total chemical synthesis of human interferon alpha-2b via native chemical ligation. J. Pept. Sci., 21 (7). S. 554 - 561. HOBOKEN: WILEY. ISSN 1099-1387

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Abstract

Interferon-alpha (IFN) is a cytokine that orchestrates innate and adaptive immune responses and potently inhibits proliferation of normal and tumor cells. These properties have warranted the use of IFN in clinical practice for the treatment of several viral infections and malignancies. However, overexpression of IFN leads to immunopathology observed in the context of chronic viral infections and autoimmune conditions. Thus, it is desirable to develop therapeutic approaches that aim at suppressing excessive IFN production. To that end, artificial evolution of peptides from phage display libraries represents a strategy that seeks to disrupt the interaction between IFN and its cell surface receptor and thus inhibit the ensuing biological effects. Mirror-image phage display that screens peptide libraries against the D-enantiomer is particularly attractive because it allows for identification of proteolysis-resistant D-peptide inhibitors. This approach, however, relies on the availability of chemically synthesized D-IFN composed entirely of D-amino acids. Here, we describe the synthesis and biological properties of IFN2b of 165 amino acid residues produced by native chemical ligation, which represents an important first step toward the discovery of D-peptide antagonists with potential therapeutic applications. Copyright (c) 2015 European Peptide Society and John Wiley & Sons, Ltd.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Li, Jing UNSPECIFIED UNSPECIFIED UNSPECIFIED Lehmann, Clara UNSPECIFIED orcid.org/0000-0002-7042-1578 UNSPECIFIED Chen, Xishan UNSPECIFIED UNSPECIFIED UNSPECIFIED Romerio, Fabio UNSPECIFIED UNSPECIFIED UNSPECIFIED Lu, Wuyuan UNSPECIFIED orcid.org/0000-0003-1318-9968 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-399897
DOI:	10.1002/psc.2760
Journal or Publication Title:	J. Pept. Sci.
Volume:	21
Number:	7
Page Range:	S. 554 - 561
Date:	2015
Publisher:	WILEY
Place of Publication:	HOBOKEN
ISSN:	1099-1387
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language SYSTEMIC-LUPUS-ERYTHEMATOSUS; D-PEPTIDE INHIBITORS; DENDRITIC CELLS; I INTERFERONS; MUTATIONAL ANALYSIS; IMMUNE-COMPLEXES; HIV-1 ENTRY; IFN-ALPHA; PROTEINS; INDUCTION Multiple languages Biochemistry & Molecular Biology; Chemistry, Analytical Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/39989