Pesch, Martina, Schultheiss, Ilka, Klopffleisch, Karsten ORCID: 0000-0002-6977-1386, Uhrig, Joachim F., Koegl, Manfred, Clemen, Christoph S., Simon, Ruediger, Weidtkamp-Peters, Stefanie and Huelskamp, Martin (2015). TRANSPARENT TESTA GLABRA1 and GLABRA1 Compete for Binding to GLABRA3 in Arabidopsis. Plant Physiol., 168 (2). S. 584 - 604. ROCKVILLE: AMER SOC PLANT BIOLOGISTS. ISSN 1532-2548

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Abstract

The MBW (for R2R3MYB, basic helix-loop-helix [bHLH], and WD40) genes comprise an evolutionarily conserved gene cassette that regulates several traits such as (pro) anthocyanin and anthocyanin biosynthesis and epidermal cell differentiation in plants. Trichome differentiation in Arabidopsis (Arabidopsis thaliana) is governed by GLABRA1 (GL1; R2R3MYB), GL3 (bHLH), and TRANSPARENT TESTA GLABRA1 (TTG1; WD40). They are thought to form a trimeric complex that acts as a transcriptional activation complex. We provide evidence that these three MBW proteins form either GL1 GL3 or GL3 TTG1 dimers. The formation of each dimer is counteracted by the respective third protein in yeast three-hybrid assays, pulldown experiments (luminescence-based mammalian interactome), and fluorescence lifetime imaging microscopy-fluorescence resonance energy transfer studies. We further show that two target promoters, TRIPTYCHON (TRY) and CAPRICE (CPC), are differentially regulated: GL1 represses the activation of the TRY promoter by GL3 and TTG1, and TTG1 suppresses the activation of the CPC promoter by GL1 and GL3. Our data suggest that the transcriptional activation by the MBW complex involves alternative complex formation and that the two dimers can differentially regulate downstream genes.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Pesch, MartinaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Schultheiss, IlkaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Klopffleisch, KarstenUNSPECIFIEDorcid.org/0000-0002-6977-1386UNSPECIFIED
Uhrig, Joachim F.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Koegl, ManfredUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Clemen, Christoph S.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Simon, RuedigerUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Weidtkamp-Peters, StefanieUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Huelskamp, MartinUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-402230
DOI: 10.1104/pp.15.00328
Journal or Publication Title: Plant Physiol.
Volume: 168
Number: 2
Page Range: S. 584 - 604
Date: 2015
Publisher: AMER SOC PLANT BIOLOGISTS
Place of Publication: ROCKVILLE
ISSN: 1532-2548
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
TRICHOME DEVELOPMENT; CELL-DIFFERENTIATION; TRANSCRIPTION FACTOR; ROOT EPIDERMIS; MYB GENES; COMPLEX; PROTEIN; FLUORESCENCE; GL3; IDENTIFICATIONMultiple languages
Plant SciencesMultiple languages
URI: http://kups.ub.uni-koeln.de/id/eprint/40223

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