Universität zu Köln

Rinschen, Markus M., Pahmeyer, Caroline, Pisitkun, Trairak, Schnell, Nicole, Wu, Xiongwu, Maass, Martina, Bartram, Malte P., Lamkemeyer, Tobias, Schermer, Bernhard ORCID: 0000-0002-5194-9000, Benzing, Thomas and Brinkkoetter, Paul T. (2015). Comparative phosphoproteomic analysis of mammalian glomeruli reveals conserved podocin C-terminal phosphorylation as a determinant of slit diaphragm complex architecture. Proteomics, 15 (7). S. 1326 - 1332. HOBOKEN: WILEY. ISSN 1615-9861

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Abstract

Glomerular biology is dependent on tightly controlled signal transduction networks that control phosphorylation of signaling proteins such as cytoskeletal regulators or slit diaphragm proteins of kidney podocytes. Cross-species comparison of phosphorylation events is a powerful mean to functionally prioritize and identify physiologically meaningful phosphorylation sites. Here, we present the result of phosphoproteomic analyses of cow and rat glomeruli to allow cross-species comparisons. We discovered several phosphorylation sites with potentially high biological relevance, e.g. tyrosine phosphorylation of the cytoskeletal regulator synaptopodin and the slit diaphragm protein neph-1 (Kirrel). Moreover, cross-species comparisons revealed conserved phosphorylation of the slit diaphragm protein nephrin on an acidic cluster at the intracellular terminus and conserved podocin phosphorylation on the very carboxyl terminus of the protein. We studied a highly conserved podocin phosphorylation site in greater detail and show that phosphorylation regulates affinity of the interaction with nephrin and CD2AP. Taken together, these results suggest that species comparisons of phosphoproteomic data may reveal regulatory principles in glomerular biology. All MS data have been deposited in the ProteomeXchange with identifier PXD001005 ().

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Rinschen, Markus M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Pahmeyer, Caroline UNSPECIFIED UNSPECIFIED UNSPECIFIED Pisitkun, Trairak UNSPECIFIED UNSPECIFIED UNSPECIFIED Schnell, Nicole UNSPECIFIED UNSPECIFIED UNSPECIFIED Wu, Xiongwu UNSPECIFIED UNSPECIFIED UNSPECIFIED Maass, Martina UNSPECIFIED UNSPECIFIED UNSPECIFIED Bartram, Malte P. UNSPECIFIED UNSPECIFIED UNSPECIFIED Lamkemeyer, Tobias UNSPECIFIED UNSPECIFIED UNSPECIFIED Schermer, Bernhard UNSPECIFIED orcid.org/0000-0002-5194-9000 UNSPECIFIED Benzing, Thomas UNSPECIFIED UNSPECIFIED UNSPECIFIED Brinkkoetter, Paul T. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-403319
DOI:	10.1002/pmic.201400235
Journal or Publication Title:	Proteomics
Volume:	15
Number:	7
Page Range:	S. 1326 - 1332
Date:	2015
Publisher:	WILEY
Place of Publication:	HOBOKEN
ISSN:	1615-9861
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language ACTIN CYTOSKELETON; PROTEIN PODOCIN; KIDNEY; COMPONENT; NEPHRIN; PODOCYTES Multiple languages Biochemical Research Methods; Biochemistry & Molecular Biology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/40331