Xiao, Lisong, Mertens, Marianne, Wortmann, Laura, Kremer, Silke, Valldor, Martin, Lammers, Twan ORCID: 0000-0002-1090-6805, Kiessling, Fabian and Mathur, Sanjay (2015). Enhanced In Vitro and In Vivo Cellular Imaging with Green Tea Coated Water-Soluble Iron Oxide Nanocrystals. ACS Appl. Mater. Interfaces, 7 (12). S. 6530 - 6541. WASHINGTON: AMER CHEMICAL SOC. ISSN 1944-8244

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Abstract

Fully green and facile redox chemistry involving reduction of colloidal iron hydroxide (Fe(OH)(3)) through green tea (GT) polyphenols produced water-soluble Fe3O4 nanocrystals coated with GT extracts namely epigallocatechin gallate (EGCG) and epicatechin (EC). Electron donating polyphenols stoichiometrically reduced Fe3+ ions into Fe2+ ions resulting in the formation of magnetite (Fe3O4) nanoparticles and corresponding oxidized products (semiquinones and quinones) that simultaneously served as efficient surface chelators for the Fe3O4 nanoparticles making them dispersible and stable in water, PBS, and cell culture medium for extended time periods. As-formed iron oxide nanoparticles (2.5-6 nm) displayed high crystallinity and saturation magnetization as well as high relaxivity ratios manifested in strong contrast enhancement observed in T-2-weighted images. Potential of green tea-coated superparamagnetic iron oxide nanocrystals (SPIONs) as superior negative contrast agents was confirmed by in vitro and in vivo experiments. Primary human macrophages (J774A.1) and colon cancer cells (CT26) were chosen to assess cytotoxicity and cellular uptake of GT-, EGCGq-, and ECq-coated Fe3O4 nanoparticles, which showed high uptake efficiencies by J774A.1 and CT26 cells without any additional transfection agent. Furthermore, the in vivo accumulation characteristics of GT-coated Fe3O4 nanoparticles were similar to those observed in clinical studies of SPIONs with comparable accumulation in epidermoid cancer-xenograft bearing mice. Given their promising transport and uptake characteristics and new surface chemistry, GT-SPIONs conjugates can be applied for multimodal imaging and therapeutic applications by anchoring further functionalities.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Xiao, LisongUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Mertens, MarianneUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Wortmann, LauraUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Kremer, SilkeUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Valldor, MartinUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Lammers, TwanUNSPECIFIEDorcid.org/0000-0002-1090-6805UNSPECIFIED
Kiessling, FabianUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Mathur, SanjayUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-408776
DOI: 10.1021/am508404t
Journal or Publication Title: ACS Appl. Mater. Interfaces
Volume: 7
Number: 12
Page Range: S. 6530 - 6541
Date: 2015
Publisher: AMER CHEMICAL SOC
Place of Publication: WASHINGTON
ISSN: 1944-8244
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
SIZE-CONTROLLED SYNTHESIS; MAGNETITE NANOPARTICLES; CONTRAST AGENTS; GRAPHENE OXIDE; RESONANCE; CANCER; ACID; FUNCTIONALIZATION; VECTORIZATION; ANTIOXIDANTMultiple languages
Nanoscience & Nanotechnology; Materials Science, MultidisciplinaryMultiple languages
URI: http://kups.ub.uni-koeln.de/id/eprint/40877

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