Universität zu Köln

Luu, Olivia, Damm, Erich W., Parent, Serge E., Barua, Debanjan, Smith, Tamara H. L., Wen, Jason W. H., Lepage, Stephanie E., Nagel, Martina, Ibrahim-Gawel, Hady, Huang, Yunyun ORCID: 0000-0001-6488-5088, Bruce, Ashley E. E. and Winklbauer, Rudolf (2015). PAPC mediates self/non-self-distinction during Snail1-dependent tissue separation. J. Cell Biol., 208 (6). S. 839 - 857. NEW YORK: ROCKEFELLER UNIV PRESS. ISSN 1540-8140

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Abstract

Cleft-like boundaries represent a type of cell sorting boundary characterized by the presence of a physical gap between tissues. We studied the cleft-like ectoderm-mesoderm boundary in Xenopus laevis and zebrafish gastrulae. We identified the transcription factor Snail1 as being essential for tissue separation, showed that its expression in the mesoderm depends on noncanonical Wnt signaling, and demonstrated that it enables paraxial protocadherin (PAPC) to promote tissue separation through two novel functions. First, PAPC attenuates planar cell polarity signaling at the ectoderm-mesoderm boundary to lower cell adhesion and facilitate cleft formation. Second, PAPC controls formation of a distinct type of adhesive contact between mesoderm and ectoderm cells that shows properties of a cleft-like boundary at the single-cell level. It consists of short stretches of adherens junction-like contacts inserted between intermediate-sized contacts and large intercellular gaps. These roles of PAPC constitute a self/non-self-recognition mechanism that determines the site of boundary formation at the interface between PAPC-expressing and -nonexpressing cells.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Luu, Olivia UNSPECIFIED UNSPECIFIED UNSPECIFIED Damm, Erich W. UNSPECIFIED UNSPECIFIED UNSPECIFIED Parent, Serge E. UNSPECIFIED UNSPECIFIED UNSPECIFIED Barua, Debanjan UNSPECIFIED UNSPECIFIED UNSPECIFIED Smith, Tamara H. L. UNSPECIFIED UNSPECIFIED UNSPECIFIED Wen, Jason W. H. UNSPECIFIED UNSPECIFIED UNSPECIFIED Lepage, Stephanie E. UNSPECIFIED UNSPECIFIED UNSPECIFIED Nagel, Martina UNSPECIFIED UNSPECIFIED UNSPECIFIED Ibrahim-Gawel, Hady UNSPECIFIED UNSPECIFIED UNSPECIFIED Huang, Yunyun UNSPECIFIED orcid.org/0000-0001-6488-5088 UNSPECIFIED Bruce, Ashley E. E. UNSPECIFIED UNSPECIFIED UNSPECIFIED Winklbauer, Rudolf UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-409799
DOI:	10.1083/jcb.201409026
Journal or Publication Title:	J. Cell Biol.
Volume:	208
Number:	6
Page Range:	S. 839 - 857
Date:	2015
Publisher:	ROCKEFELLER UNIV PRESS
Place of Publication:	NEW YORK
ISSN:	1540-8140
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language CONVERGENT EXTENSION MOVEMENTS; CELL-ADHESION; XENOPUS GASTRULATION; VERTEBRATE GASTRULATION; PARAXIAL PROTOCADHERIN; ZEBRAFISH GASTRULATION; REGULATES GASTRULATION; EMBRYONIC-DEVELOPMENT; CADHERIN ADHESION; SIGNALING PATHWAY Multiple languages Cell Biology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/40979