Universität zu Köln

Kehrein, Kirsten, Schilling, Ramon, Moeller-Hergt, Braulio Vargas, Wurm, Christian A., Jakobs, Stefan ORCID: 0000-0002-8028-3121, Lamkemeyer, Tobias, Langer, Thomas and Ott, Martin ORCID: 0000-0001-6367-3091 (2015). Organization of Mitochondrial Gene Expression in Two Distinct Ribosome-Containing Assemblies. Cell Reports, 10 (6). S. 843 - 854. CAMBRIDGE: CELL PRESS. ISSN 2211-1247

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Abstract

Mitochondria contain their own genetic system that provides subunits of the complexes driving oxidative phosphorylation. A quarter of the mitochondrial proteome participates in gene expression, but how all these factors are orchestrated and spatially organized is currently unknown. Here, we established a method to purify and analyze native and intact complexes of mitochondrial ribosomes. Quantitative mass spectrometry revealed extensive interactions of ribosomes with factors involved in all the steps of posttranscriptional gene expression. These interactions result in large expressosome-like assemblies that we termed mitochondrial organization of gene expression (MIOREX) complexes. Superresolution microscopy revealed that most MIOREX complexes are evenly distributed throughout the mitochondrial network, whereas a subset is present as nucleoid-MIOREX complexes that unite the whole spectrum of organellar gene expression. Our work therefore provides a conceptual framework for the spatial organization of mitochondrial protein synthesis that likely developed to facilitate gene expression in the organelle.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Kehrein, Kirsten UNSPECIFIED UNSPECIFIED UNSPECIFIED Schilling, Ramon UNSPECIFIED UNSPECIFIED UNSPECIFIED Moeller-Hergt, Braulio Vargas UNSPECIFIED UNSPECIFIED UNSPECIFIED Wurm, Christian A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Jakobs, Stefan UNSPECIFIED orcid.org/0000-0002-8028-3121 UNSPECIFIED Lamkemeyer, Tobias UNSPECIFIED UNSPECIFIED UNSPECIFIED Langer, Thomas UNSPECIFIED UNSPECIFIED UNSPECIFIED Ott, Martin UNSPECIFIED orcid.org/0000-0001-6367-3091 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-412322
DOI:	10.1016/j.celrep.2015.01.012
Journal or Publication Title:	Cell Reports
Volume:	10
Number:	6
Page Range:	S. 843 - 854
Date:	2015
Publisher:	CELL PRESS
Place of Publication:	CAMBRIDGE
ISSN:	2211-1247
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language SACCHAROMYCES-CEREVISIAE MITOCHONDRIA; CYTOCHROME-B SYNTHESIS; PROTEIN-SYNTHESIS; TRANSLATIONAL ACTIVATOR; FUNCTIONAL INTERACTIONS; MAMMALIAN MITOCHONDRIA; MEMBRANE ORGANIZATION; YEAST MITOCHONDRIA; INNER MEMBRANE; RNA GRANULES Multiple languages Cell Biology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/41232