Universität zu Köln

Luebke, Nadine, Di Cristanziano, Veronica, Sierra, Saleta, Knops, Elena, Schuelter, Eugen, Jensen, Bjorn, Oette, Mark, Lengauer, Thomas ORCID: 0000-0003-3801-2640 and Kaiser, Rolf (2015). Proviral DNA as a Target for HIV-1 Resistance Analysis. Intervirology, 58 (3). S. 184 - 190. BASEL: KARGER. ISSN 1423-0100

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Abstract

Background: Resistance analysis from viral RNA is restricted to detectable viral load. Therefore, analysis from proviral DNA could help in cases with low-level or suppressed viremia. Methods: Viral plasma RNA and the corresponding cellular proviral DNA of 78 EDTA samples from 48 therapy-naive (TN) and 30 therapy-experienced (TE) HIV-1-infected patients were isolated and analyzed for their resistance profiles in the protease and reverse transcriptase genes. Results: Overall, 175 drug-resistance mutations (DRMs) were detected in 25/30 TE (83.3%) and 5/48 TN (10.4%) samples. The TE patients displayed a mean number of 6.68 DRMs in RNA and 5.20 in DNA. In the TN patients, a mean of 0.8 DRMs was found in RNA and 1.0 in DNA; 75% of the DRMs were detected in RNA and DNA simultaneously. In the TE samples, 76% of the DRMs were detected simultaneously in RNA and DNA, 23% exclusively in RNA and 1% in DNA only. The TN samples revealed a significantly higher frequency of DRMs in DNA than in RNA. Conclusions: Proviral DNA resistance testing provides additional resistance information for TN patients. It is also a reliable alternative for TE patients with unsuccessful RNA testing and can provide valuable information when no records are available. (C) 2015 S. Karger AG, Basel

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Luebke, Nadine UNSPECIFIED UNSPECIFIED UNSPECIFIED Di Cristanziano, Veronica UNSPECIFIED UNSPECIFIED UNSPECIFIED Sierra, Saleta UNSPECIFIED UNSPECIFIED UNSPECIFIED Knops, Elena UNSPECIFIED UNSPECIFIED UNSPECIFIED Schuelter, Eugen UNSPECIFIED UNSPECIFIED UNSPECIFIED Jensen, Bjorn UNSPECIFIED UNSPECIFIED UNSPECIFIED Oette, Mark UNSPECIFIED UNSPECIFIED UNSPECIFIED Lengauer, Thomas UNSPECIFIED orcid.org/0000-0003-3801-2640 UNSPECIFIED Kaiser, Rolf UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-416166
DOI:	10.1159/000431093
Journal or Publication Title:	Intervirology
Volume:	58
Number:	3
Page Range:	S. 184 - 190
Date:	2015
Publisher:	KARGER
Place of Publication:	BASEL
ISSN:	1423-0100
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Mathematics and Computer Science > Institute of Computer Science
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language LOW-LEVEL VIREMIA; ACTIVE ANTIRETROVIRAL THERAPY; BLOOD MONONUCLEAR-CELLS; GENOTYPIC RESISTANCE; EXPERIENCED PATIENTS; MUTATIONS; PLASMA; NAIVE; EVOLUTION; RESERVOIR Multiple languages Virology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/41616