Universität zu Köln

Kukuk, Damaris and Schildgen, Oliver ORCID: 0000-0003-4297-9627 (2015). Isolation of nascent DNA fragments from cells synchronously infected with HSV-1 reveals bidirectional initiation of replication at oriL. Future Virol., 10 (3). S. 211 - 220. LONDON: FUTURE MEDICINE LTD. ISSN 1746-0808

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Abstract

Background: How HSV-1 DNA replication is initiated in infected cells is not fully understood. Experiments with temperature-sensitive HSV mutants have shown that DNA replication is a biphasic process that initially depends on the origin binding protein. Aims: The aim of the study was to answer the question at which origin of replication the HSV-1 DNA replication starts in the infected cell. Methods: Using the tsS mutant the HSV-1 infection was synchronized and newly synthesized nascent DNA fragments were analysed. Results: Nascent viral DNA was observed predominantly around the oriL, giving raise to the hypothesis that the replication starts at this origin in vivo. Conclusion: We show for the first time that HSV-1 DNA replication begins exclusively at the oriL site and proceeds in a bidirectional manner.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Kukuk, Damaris UNSPECIFIED UNSPECIFIED UNSPECIFIED Schildgen, Oliver UNSPECIFIED orcid.org/0000-0003-4297-9627 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-416941
DOI:	10.2217/FVL.14.112
Journal or Publication Title:	Future Virol.
Volume:	10
Number:	3
Page Range:	S. 211 - 220
Date:	2015
Publisher:	FUTURE MEDICINE LTD
Place of Publication:	LONDON
ISSN:	1746-0808
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language HERPES-SIMPLEX-VIRUS; ORIGIN-BINDING-PROTEIN; CHAIN GROWTH; POSSIBLE DISCONTINUITY; ESCHERICHIA-COLI; MECHANISM; PIECES; RECOMBINATION; LOCALIZATION; SEQUENCE Multiple languages Virology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/41694